3 - Erythropoiesis and Approach to Anemia Flashcards
gene that enables differentiation into RBCs and megakaryocytes/platelets
GATA-1
how long does it take to make a mature RBC?
28 d
RBC precursors
Pronormoblast Basophilic normoblast Polychromatophilic normoblast Orthochromatic normoblast Reticulocyte Mature RBC
what stimulus is detected by the kidney to induce EPO production and release?
low blood oxygenation
secondary site of EPO production
liver (10%)
type of anemia caused by renal insufficiency
normochromic normocytic
what specifically does EPO do to increase RBC production?
prevents apoptosis of RBC precursors
4 non-EPO stimulators of erythrogenesis
GM-CSF, IL-3, insulin like growth factor, androgens
3 inhibitors of erythrogenesis
IFN gamma, IL1, TNF
what makes Darbepoietin different than other recombinant EPOs?
additional glycosylation site > longer half life
what protein detects low blood oxygenation to stimulate EPO production?
HIFalpha
what protein is responsible for the constitutive release of EPO?
HIFbeta
what disease can interfere with HIFalpha signaling / EPO regulation?
von Hippel Lindau syndrome - it is needed for degradation of HIFalpha when oxygenation is high
EPO receptor is member of what receptor family
cytokine
polycythemia vera
mutation of JAK2 on EPO receptor results in spontaneous dimerization > increased erythropoiesis in absence of EPO