3. Cell Membrane Flashcards
The resolution limit for light microscope is ____?
0.1um
“unit” membrane concept
-is trilaminar, consisting of protein and lipid
What chemical is used to fix plasma membranes?
OsO4
Osmium tetraoxide interact with the _______ portions of the phospholipid molecules of the unit membrane.
Hydrophilic
What are the 3 main classes of lipids in cell membrane?
- Phospholipids
- Sphingolipids
- Cholesterol
Glycolipids are positioned where on the cell membrane?
-only within the outer of the lipid bilayers with their carbohydrate moieties exposed on the cell surface.
What type of protein can be easily extracted with a salt solution?
peripheral proteins
What type of protein needs a detergent to be extracted from the cell membrane?
Integral proteins
Ion channels are formed by what type of protein?
Multi-pass integral protein
Receptors are typically formed by what type of protein?
Single-pass integral protein
Can lipids move in the plane of the membrane? Does it require energy?
Yes! But it does NOT require energy.. Simple diffusion or Brownian motion.
Does movement of a lipid from one leaflet to another require energy?
Yes
______ facilitate ATP dependent movement of a lipid from one leaflet to the other.
Flippases
Do membrane proteins connect to microtubules?
NO!
The membrane proteins that are anchored to the cytoskeleton use adaptor proteins like _______ to then connect to actin microfilaments.
ankyrin
Can proteins flip-flop in the membrane?
No
(T/F) Most membrane proteins are restricted in their lateral diffusion by attachment to cytoskeletal componets and membrane features.
True
______ are elongated motile processes with a highly organized microtubluar core.
Cilia