3 & 4. Electronic Spectroscopy Flashcards
what is a chromophore?
A Chromophore is the part of the molecule containing the orbitals involved in the transition
what do electronic transitions classify?
absorptions according to the nature of the excited state
what is a bound - bound transition?
where both states are stable and spectral features narrow to show vibrational structure
what is a bound - free transition? 2 points
when the exited state dissociates, spectrum is broad
label the components of the absorbtion spectrophotometer
what is structural colour
sturctural colour is caused by interference effects - not pigments.
its a nanostructure that reflects/ scatters light so the waves of certain frequencies can constructuively interfere
give four benefits of using a dual beam specrometer design
- they sample and refrence channels simultaneously measured
- dont need to reomve the sample to get refrence
- compensation for variaton of light intensity
- compensates for absorbtion of cell
- compenstaes for aborbtion of solved
how can fluorescene be used as a qualitive analytical tool - give an example
fluorescene can be used for use of GFP. can use a GFP chromophore to see your gene of interest
how does bioluminecene work?
it is the production and emission of light by a living organsim.
its a form of chemiluminesce
it works by exciting ketone bodies
how does iron catalyse the reaction for chemluminese?
iron catalyses the intersystem crossing this give more light. oxygen + iron (iron is the catalyst) gives a strong light
what is strokes raman scattering? (hint, its a type of inelastic light)
Scattered light loses energy equivalent to molecule vibration
look at image
what is anti strokes raman scattering?
Scattered light gains energy equivalent to molecule vibration
