29 Chromatography And Spectroscopy

Question 1

What are the 2 forms of chromatography?

Answer 1

• stationary phase

- mobile phase

Question 2

What happens in the stationary phase?

Answer 2

Stationary phase is a solid or liquid that does not move supported on a solid

Question 3

What happens in the mobile phase?

Answer 3

Does move and is normally a liquid or a gas

Question 4

What applications does chromatography have?

Answer 4

analysis of:

• drugs
• plastics
• flavourings
• air samples

Question 5

What does thin layer chromatography indicate?

Answer 5

Indicates how many components are in a mixture

Question 6

Give an example of a TLC plate

Answer 6

• Plastic sheet

- Glass

Question 7

What is the adsorbent substance usually?

Answer 7

Silica

Question 8

In TLC the adsorbent is in which phase?

Answer 8

Stationary

Question 9

Adsorption definition

Answer 9

Process by which the solid silica holds the different substances in the mixture to its surface

Question 10

How is separation of the mixture achieved in TLC?

Answer 10

The relative adsorption of substances with the stationary phase

Question 11

What is the highest Rf value that can be achieved?

Answer 11

1

Question 12

Effect value formula

Answer 12

Rf = distance moved by the component/ distance moved by the solvent front