2.6 Fermintation Flashcards
When do we use fermentation?
When no oxygen is present
a mixture of lactic acid, ethanol and/or acetic acid, and CO2 as a result, because of their use of the branched pentose phosphate pathway instead of the EMP pathway for glycolysis. One
heterolactic fermentation,
Occurs in yeast and some bacteria
Produces ethanol and CO2
Used to produce beer and wine
Microbes die off as ethanol concentrations reach 12 to 15%.
Alcoholic Fermentation
NADH from glycolysis is oxidized
Pyruvate or a derivative accepts electrons
ATP is formed only by substrate-level phosphorylation during glycolysis
Oxidative phosphorylation does not occur
Fermentation
It generates both lactic acid AND ethanol.
Carried out by the LAB Leuconostoc
Heterolactic Fermentation
Carried out by Gram-positive microbes known as “lactic acid bacteria” (LAB)
Includes pathogens, GI and GU tract normal flora, and cultures used to make yogurt
Homolactic Fermentation
Mixture of chemicals produced
Occurs in several bacteria and fungi
Used for the commercial production of chemicals such as acetone and butanol
Mixed Acid Fermentation
Proteases: hydrolyze polypeptides to amino acids
Individual amino acids have the amino group detached, leaving an organic acid that can catabolized through fermentation or the TCA Cycle
Protein Catabolism
Triglycerides are hydrolyzed by lipases to:
Glycerol: converted to DHAP (degraded via glycolytic pathway)
Fatty Acids: often oxidized via β-oxidation pathway to form acetyl CoA
Lipid Catabolism