2.3 Culturing Microorganisms

Question 1

What is a liquid media?

Answer 1

solution of beef extract, peptone, yeast extract, and/or other nutrient material

Question 2

What is a semisolid media?

Answer 2

liquid media containing 0.5% agar; used to determine motility of bacterial species

Question 3

What is a solid media?

Answer 3

nutrient material with 1.5% or higher of agar

Question 4

What is a complex media?

Answer 4

contain some ingredients of unknown composition and/or concentration

Question 5

What is a chemically defined media?

Answer 5

all compounds and their concentrations are known

Question 6

What is a supportive (general purpose) media?

Answer 6

a mixture of nutrients that will support the growth of most microbes

Question 7

What is a enrichment media?

Answer 7

supplemented by blood or other special nutrients

Question 8

What is Selective media?

Answer 8

favor the growth of some and inhibit growth of others

Question 9

What is differential media?

Answer 9

cause an observable change that distinguishes between different types of microbes

Question 10

What is FISHing

Answer 10

Sequences can be used to produce fluorescent probes that will bind to complementary DNA (fluorescent in situ hybridization or FISH).

Question 11

Luria Agar (LA)

Answer 11

Enrichment

Question 12

Mannitol-Salt Agar

Answer 12

Differential, Selective

Question 13

Spirit Blue Agar

Answer 13

Differential

Question 14

Starch Agar

Answer 14

Differential

Question 15

Hektoen Enteric (HE) Agar

Answer 15

Differential, Selective

Question 16

MacConkey’sAgar

Answer 16

Differential, Selective

Question 17

Blood Agar

Answer 17

Enrichment, Differential

Question 18

Chocolate Agar

Answer 18

Enrichment