25. Immunoproliferative Diseases

Question 1

Suppose a serum sample run on an IFE gel produces monoclonal bands in the IgG and kappa lanes. What disease is associated with this pattern?

Answer 1

Multiple myeloma

Question 2

Suppose a serum sample run on an IFE gel produces monoclonal bands in the IgM and lambda lanes. What disease is associated with this pattern?

Answer 2

Waldenstrom’s macroglobulinemia

Question 3

Suppose a serum sample run on an IFE gel produces a monoclonal band in the kappa lane only. What type of protein is this? What disease is associated with this pattern?

Answer 3

• Bence jones protein

- Multiple myeloma

Question 4

What is the difference in appearance between monoclonal and polyclonal bands on an IFE gel?

Answer 4

Monoclonal = narrow band due to homogenous nature
Polyclonal = diffuse band that migrate to different positions

Question 5

How are cryoglobulins different from cold agglutinins?

Answer 5

Cryoglobulins = immunoglobulins themselves precipitate below 37 degrees celsius
Cold agglutinins = cold causes agglutination of red blood cells

Question 6

What type of cryoglobulin consists of polyclonal IgG plus a monoclonal immunoglobulin? What kinds of diseases is this type of cryoglobulin associated with?

Answer 6

Type II (2) - Mixed cryoglobulins

Question 7

How will cryoglobulins appear at 4 degrees C and at 37 degrees C?

Answer 7

37 degrees = No precipitate should form

4 degees = precipitate should be visible