2.3 Purifying DNA Experiment Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q
  1. Break up cells using a …
A

Blender

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q
  1. Make solution what should it contain?
A

Detergent
Salt
Distilled water

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q
  1. What do you add to the detergent solution in a beaker?
A

The broken up cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q
  1. Now you incubate the solution, at what temperature and how long for?
A

60 degrees

15 minutes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What does the detergent in the mix do?

A

Breaks down cell membranes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What does the salt in the solution do?

A

Binds to DNA causing it to clump

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What does the temperature of the water bath do?

A

Stops enzymes working and breaking down the DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q
  1. Now what do you put the mixture in? And what do you then do?
A

Ice bath to cool

Once cooled filter the mixture and transfer sample into clean boiling tube

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q
  1. Next add protease enzymes, what do they do?
A

Break down proteins in mixture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q
  1. Also add RNAse enzyme, what will this do?
A

Break down any RNA in mixture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q
  1. Now what do you dribble down the side of the tube and what does it do?
A

Cold ethanol

Forms layer on top of mixture

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q
  1. Leave for a few minutes and what happens?
A

DNA forms white precipitate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly