2.3 Purifying DNA Experiment Flashcards
1
Q
- Break up cells using a …
A
Blender
2
Q
- Make solution what should it contain?
A
Detergent
Salt
Distilled water
3
Q
- What do you add to the detergent solution in a beaker?
A
The broken up cells
4
Q
- Now you incubate the solution, at what temperature and how long for?
A
60 degrees
15 minutes
5
Q
What does the detergent in the mix do?
A
Breaks down cell membranes
6
Q
What does the salt in the solution do?
A
Binds to DNA causing it to clump
7
Q
What does the temperature of the water bath do?
A
Stops enzymes working and breaking down the DNA
8
Q
- Now what do you put the mixture in? And what do you then do?
A
Ice bath to cool
Once cooled filter the mixture and transfer sample into clean boiling tube
9
Q
- Next add protease enzymes, what do they do?
A
Break down proteins in mixture
10
Q
- Also add RNAse enzyme, what will this do?
A
Break down any RNA in mixture
11
Q
- Now what do you dribble down the side of the tube and what does it do?
A
Cold ethanol
Forms layer on top of mixture
12
Q
- Leave for a few minutes and what happens?
A
DNA forms white precipitate
