2.1.2 Slides and photomicrographs

Question 1

how are slides prepared

Answer 1

dehydrate specimen, embbed the spiceim in wax to prevent distorion during slicing, these are then sliced, stained and mounted in chemicals to perserve them.

Question 2

“state the term used to staining specimens to see certian components
“

Answer 2

differencial staining

Question 3

how could you look at a colourless specimen

Answer 3

uses are dark background works for illuminated speicimens, or you can adjusting the iris diaphram ro reduce illumination

Question 4

“what stians the cytoplasm

“

Answer 4

eosin

Question 5

what stains starch and cellulose

Answer 5

iodine in potasium iodine

Question 6

what stains lipids

Answer 6

sudan red

Question 7

“what stains DNA/chromosomes dark red

“

Answer 7

acetic orcein

Question 8

What is the name of the thin sheet of glass which covers the specimen and which is found on top of a microscope slide? (1)

Answer 8

Cover slip

Question 9

What stain did we use for viewing onion cells?(1)

Answer 9

Iodine in potassium iodide

Question 10

What is micrograph?(1)

Answer 10

Image taken by a microscope

Question 11

What stain might bind to the plasma membrane?(1)

Answer 11

Sudan red (as this part is made of phospholipids)

Question 12

What is the equation for calculating magnification?(1)

Answer 12

M = I/A

Question 13

Convert 3.25 mm into micrometres(1)

Answer 13

3.25mm x 1000 = 3250 micrometres

Question 14

The part of the microscope you look through is called?(1)

Answer 14

Eyepiece lens

Question 15

The part of the microscope that you put the slide on is called? (1)

Answer 15

Stage

Question 16

Name a stain that will bind to chromosomes or DNA? (1)

Answer 16

Acetic orcein

Question 17

Name a stain that will bind to lipids?

Answer 17

Sudan red

Question 18

Name a stain that will bind strongly to the nucleus than compared to the rest of the cell?

Answer 18

Methylene blue

Question 19

Name a stain that binds to the cytoplasm?

Answer 19

Eosin

Question 20

What word is being defined – A process which uses stains to by to specific structures in cells so they can easily be identified? (1)

Answer 20

Differential staining