2.1 Microscopy Flashcards

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1
Q

How many lenses does a light microscope have, name them? (2 marks)

A

2- eyepiece lens, objective lens

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2
Q

How is resolution adjusted using a light microscope? (2 marks)

A

Fine focusing knob
Coarse focusing knob

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3
Q

Name 4 ways to prepare a sample to view under a microscope. (4 marks)

A

Dry mount
Wet mount
Squash slices
Smear slides

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4
Q

Describe a dry mount. (2 marks)

A

Solid specimens cut into very thin slices.
Placed on slide and a cover slip is placed over the sample.

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5
Q

Describe a wet mount. (2 marks)

A

Specimens are suspended in liquid.
A cover slip is placed on from an angle.

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6
Q

Describe a squash slide. (2 marks)

A

Wet mount prepared first.
Then lens tissue is used to press down on the cover slip.

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7
Q

Describe a smear slide. (2 marks)

A

Edge of a slide is used to smear the sample.
A cover slip is placed over the sample.

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8
Q

Why are stains sometimes used when viewing samples? (2 marks)

A

Increase contrast of components.
Allows components to become visible so they can be identified.

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9
Q

How does gran staining work? (2 marks)

A

Gram positive bacteria retain the stain.
Gram negative bacteria have thinner cell walls so they lose the stain.

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10
Q

Why is acid-fast staining used? (1 mark)

A

Used to differentiate between sources of Mycobacterium.

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11
Q

Explain how a student can add a stain to a wet mount slide. (2 marks)

A

Put a drop of stain next to one edge of the cover slip and a piece of paper towel next to opposite edge.
The paper towel will draw the stain under the slip.

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12
Q

What two stages are needed to prepare a large tissue specimen for light microscopy? (2 marks)

A

Sectioning
Staining

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13
Q

Why do most specimens need to be stained? (1 mark)

A

Many cells are colourless, so detail cannot be seen unless stained.

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