2. Replication of DNA - Polymerase Chain Reaction Flashcards

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1
Q

What is PCR a technique for?

A

The amplification of DNA in vitro

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2
Q

What are the primers used complementary to?

A

Specific target sequences at the two ends of the region to be amplified

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3
Q
  1. Why is DNA heated?
A

To separate the strands

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4
Q
  1. What does cooling allow?
A

Primers to bind to their complementary target seuences

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5
Q
  1. What do the primers allow?
A

DNA polymerase to start the replication of the region

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6
Q
  1. The DNA polymerase is…
A

Heat tolerant (it is DNA polymerase from an extremophile bacterium, thermus aquaticus)

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7
Q
  1. What happens to the number of copies of the region of DNA after each cycle?
A

It doubles

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8
Q
  1. How is amplification achieved?
A

By repeated cycles of heating and cooling

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9
Q

What is a negative control used to do?

A

Detect contamination

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10
Q

What is a positive control used to do?

A

Detect when a reaction fails due to a problem with the thermocycler or a reaction component

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