19.07.16 Databases and workflow Flashcards
1
Q
What are databases for
A
-Sample tracking, manage workload, record results
2
Q
What is LIMS
A
Laboratory Information Management System
3
Q
Benefits of LIMS
A
- Multiple users can access and update information
- Prevent duplicate testing if multiple samples arrive
- Provide statistics such as TATs, volume of samples received (other performance indicators)
- Finance
- Link to lab equipment (worksheets etc), less manual transcription, which could lead to errors.
- Reduces paper usage
4
Q
Issues with information governance and LIMS
A
- All users should their own login, can track actions also ensures only correct individuals can access data
- Data should be backed up regularly and be secure
5
Q
Purpose of internal disease specific databases
A
- To track patient samples from receipt to reporting.
- To record variants identified (aids analysis in future patients)
6
Q
Use of primer databases
A
- Lots of primers are generated to confirm NGS findings. Need to be recorded.
- can check if we have primers for new variants so as not to duplicate work
7
Q
Examples of QC databases
A
- Q-pulse
- iPassport
8
Q
Purpose of qc databases
A
- To store electronic documents (active, archived SOPs), risk assessments etc
- MSDS for reagents, track consumables, batch numbers etc
- Inventory of equipment in lab, store maintenance records and service contracts
- Log non-conformances
- Provide a system for auditing
- Staff training record, Annual leave monitoring to ensure cover over busy times (Christmas)
9
Q
How long does the RCPath (2015) guidelines state that data should be stored for use in family studies
A
30 years
10
Q
Issues with NGS data storage
A
- Raw files are very large
- Analysis methods changing continuously making repeat analysis of historical data difficult (almost easier to re-sequence)
- NHS IT infrastructure is often inadequate
11
Q
-Purpose of IT databases in a lab
A
- allows for an efficient workflow in the lab.
- Easier and more accurate testing (e.g. use of barcodes) and monitoring of progress and results.
- Reduce errors (compared to manual data entry).
