1.8 factors effecting enzyme action Flashcards
Hep does temperature help increase the rate of reaction
This is because the thermal energy turns to kinetic energy as a result molecules have more successful collision meaning more enzyme substrate complex can be formed
How does temperature decrease rate of reaction
Rise in temperature causes hydrogen and other bonds in an enzyme molecule to break. The active site changes shape and the substrate can not fit into the active site as easily. When the substrate can no longer fit into the changed active site it is called denaturing.
What is the optimum temperature for enzymes in human body
40°C
How does pH affect rate of reaction
If pH is not optimum the rate of reaction will decrease however if the change is drastic the enzyme will become denatured
How does pH affect a enzyme
A change in pH alters the charges on the amino acids in the active site therefore ESC( enzyme substrate complex) can no longer be formed
What happens if enzyme concentration is increased
At first if there is an excess of substrate the increase of enzyme concentration will be proportional to rate of reaction but if substrate is limiting factor then the rate of reaction will not increase even if enzyme concentration does
What happens when substrate concentration is increased
At first the rate of reaction will increase because the enzymes will begin to work at full capacity but when they are working at full capacity an increase in substrate concentration will not affect the rate of reaction