16.1-16.3 Flashcards

1
Q

Stress response systems help bacteria

A

adapt and survive under challenging conditions

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2
Q

Disrupt important physiological processes such as DNA replication and cell division

A

pathogens

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3
Q

Bacterial chromosome

A

densely packed structure (nucleoid body)

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4
Q

Bacterial cell division takes place via

A

binary fission

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5
Q

On average, an E. coli genome has ~

A

4700 genes

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6
Q

Core genome

A

encodes genes important for metabolic functions of E.
coli species (cell membranes, cell wall . . .)

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7
Q

Variable genome

A

encodes genes necessary for adaptation and survival
in diverse environments

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8
Q

Pangenome

A

Genes from core genome plus all the genes from different
strains of a given bacterium

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9
Q

Bacterial genomes contain

A

DNA transpoons

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10
Q

With IS, enzyme transposase is flanked by

A

repeat sequences at the ends of the IS element

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11
Q

With IS, Transposase enzyme recognizes the inverted repeats, and

A

catalyzes the excision and insertion of the transposon sequence

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12
Q

Composite transposable (Tn) elements contain

A

resistance genes against antibiotics or toxic metals in addition to transposase

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13
Q

insertion of a transposon within genes can cause

A

mutations or affect gene regulation

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14
Q

Transposons can cause

A

genomic rearrangements

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15
Q

Transposon distribution within the bacterial genome can

A

vary
widely between strains of the same species

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16
Q

Plasmids consist of

A

extrachromosomal DNA

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17
Q

F episome participates in gene transfer via

A

conjugation

18
Q

episome compared to plasmid

A

episome much larger and can integrate into the
chromosome

19
Q

transposons carry the

A

the antibiotic resistance genes

20
Q

Plasmids can be transferred to other

A

teria, sometimes across species

21
Q

Plasmids being transferred can lead to

A

rapid spread of antibiotic resistance

22
Q

Multiple resistance genes may be located between

A

two IS elements, allowing them to transpose as a unit

23
Q

If pathogenic bacteria acquire the plasmids, they can become resi

A

antibiotics

24
Q

Auxotrophic mutations

A

cannot grow in minimal medium

25
Q

Mutations affecting catabolism resulting in

A

an inability to break
down complex compounds/chemicals

26
Q

Classes of bacterial mutants

A

Prototroph and Auxotroph

27
Q

Prototroph mutants can synthesize

A

all of its amino acids, nucleic acids, vitamins, and other
cellular constituents from inorganic nutrients

28
Q

Prototroph can grow in

A

minimal medium or medium lacking the supplement

29
Q

A Selection

A

a growth condition that allows for the selective
propagation of genetically marked cells

30
Q

A Screen

A

growth condition where both mutant and wild type are
able to grow but can be distinguished phenotypically

31
Q

Any mutation of the leu gene will result in

A

bacteria being auzotroph for leucine (unable to synthesize leucine and can only grow in media supplemented with leucine)

32
Q

Horizontal gene transfer

A

A process by which transfer of genetic material occurs between two organisms
without one being its offspring

33
Q

Horizontal gene transfer is important

A

for bacterial evolution and contributes to the spread of antimicrobial
resistance in bacteria

34
Q

Conjugation horizontal gene transfer

A

Donor DNA is transferred directly to recipient through a connecting tube. Contact and transfer are promoted by a specialized plasmid in the donor cell

35
Q

Transduction horizontal gene transfer

A

Donor DNA is packaged in released bacteriophage

36
Q

Transformation horizontal gene transfer size

A

~500 bp - 10 kb

37
Q

Transformation horizontal gene transfer creates

A

transformant

38
Q

Conjugation horizontal gene transfer size of DNA segment

A

~30-50 kb

39
Q

Conjugation horizontal gene transfer size of DNA mediated by special structures

A

Conjugative pili and Encoded by the F plasmid

40
Q

Transduction horizontal gene transfer size of DNA segment

A

~40 - 120 kb

41
Q

Transduction horizontal gene transfer creates

A

transductant

42
Q

Conjugation horizontal gene transfer creates

A

Exconjugant