14 - Skeletal Muscle Flashcards
What is muscle? What are the different types?
One of the four “basic” tissues (nervous, epithelial, CT)
Skeletal, cardiac, and smooth - prefixes myo- and sacro-
Skeletal = voluntary
Cardiac and smooth = involuntary
What are skeletal muscle cells called? How big are they?
Aka fibers or myocytes
Largest cell - similar but not identical to cardiac myocytes. Have 100s of nuclei at cell periphery.
Describe the structure of skeletal muscle?
Highly structured, in a heirarchical fashion.
Gross muscle (cm) > fascicles (mm) > myocytes (10-100 microm) > myofibrils (~1 microm) > myofilament
Describe the epimysium, perimysium, and endomysium (CT investing in skeletal muscle).
Epimysium: deep fascia, invests the entire muscle
Perimysium: invests fascicles (groups of myocytes); continuous with CT at the myotendon junction
Endomysium: invests individual myocytes; adjacent to basal lamina.
What do you see on light microscopy of four adjacent myocytes?
- Nuclei are peripheral
- Striated: alternating A bands (dark) And I bands (light)
- Z lines demarcate the sarcomere (unit of contraction)
Myocytes contain ______ that contain __________.
Myocytes contain myofibrils, that are adjacent to each other and aligned in register.
Each myofibril (~1 microm) is composed of thick and thin myofilaments (nm).
What are the different bands and zones seen in a myofibril?
I-band (light band) is bisected by the Z line.
A-band (dark band) is bisected by the H-zone/M line (contains MM-CK)
Sarcomeres (~2 microm long) reside between adjacent Z lines.
The ____ is the unit of striated muscle contrastion.
The sarcomere.
What are the two membranes of skeletal myocytes?
T (transverse) tubules: invaginate sarcolemma
SR (sarcoplasmic reticulum): envelops each myofibril
What is the structure of the presynaptic terminal, the cleft, and postsynaptic terminal of the NMJ?
Presynaptic: Ca2+ channels, synaptic vesicles (with ACh)
Cleft: AChE
Postsynaptic: junctional folds, AChRs facing cleft, Na+ channels in folds
What occurs at the neuromuscular (myoneural) junction?
- Nerve AP causes Ca2+ to enter the neuron.
- Synaptic vesicles fuse to pre-synaptic membrane and ACh is released into cleft
- ACh binds AChR on myocyte and Na+ enters the yocyte to cause a muscle AP.
What can block all steps at the NMJ that occur prior to Na+ enterings the myocyte to cause a msucle AP?
Succinylcholine or curariform drugs
What occurs during skeletal muscle excitation?
Depolarization in the T tubules causes calcium channel Cav 1.1 to be released.
Cav1.1 binds RyR (receptor) in the SR membrane, causing a huge efflux of Ca2+ from the sarcoplasmic reticulum into the cytoplasm.
Now the contraction phase can begin.
What occurs during skeletal muscle contraction?
- Ca2+ binds troponin C and tropomyosin moves out of the way.
- ATP > ADP + Pi and myosin binds actin
- Power stroke = contraction: thin filaments move into A-band and the sarcomere shortens
What moves to cause sarcomere shortening? What doesn’t change during contraction?
Thin filaments move.
During contraction: A-band is unchanged while I-band is shortened.