14. phagocytosis Flashcards
is the cellular process of engulfin solid particles by the cell membranes to form an internal phagosome by phagocytes
phagocytosis
who revelead that the phagocytosis is a specific form of endocytosis involving the vesicular internalization of solid
ilya Mechnikov 1882
__ in involved in the acquisition of nutrients for some cells, and, in the immune system, it is a major mechanism used to remove pathogens and cell debris
phagocytosis
several types of cells in the immune system engulf microorganism via __
phagocytosis
_ are abundant in the blood, quickly enter tissues, and phagocytize pathogens in acute inflammation
neutrophils
_are closely related to monocytes in the blood. Longer-lived cells predominate in chronic inflammation
Macrophages
___ is a small amount of phagocytosis in these cells is often necessary in order for them to develop into cells that release antibodies
B lymphocytes
where does phagocytosis begins
begins with the neutrophils or macrophage flowing around the pathogen and engulfing it
where does phagocytosis begins
begins with the neutrophils or macrophage flowing around the pathogen and engulfing it
2nd step of phagocytosis
fusion of lysosomes (phagolysosomes)
where does the lysosomes derived from
q
golgi apparatus
materials
glass slide
sterile needle and syringe
EDTA tube
innoculating loop
bacterial broth culture consisting of a muxture of bacteria
alcohol lamp
hema-quick stain
pasteur piptte
water bath
Procedure:
- Collect 3ml of venous blood in EDTA tube using aseptic technique
- Add 2-3 drops of bacterial suspension and mix by gentle shaking
- Incubate the tube at 37C in a water bath for 30 mins
- Centrifuge the tube for 5minutes
- Using pasteur pipette, make a smear in a glass slide
- Stain the smear using hema-quick stain
- Examine under oil immersion objective for the presence of micro organisms within the leukocytes
Phagocytosis in these cells is important for the elaboration of a specific immune response rather than for directly destroying the pathogens
Dendritic cells
3 steps of phagocytosis
Recognition and adherence
Engulment
Intracellular killing