11. Laboratory techniques in mycology Flashcards
essential components of ecosystem and are widely distributed
fungi
they are a large group eukaryotic organisms whose main role in nature is to break down nonliving organic materials in order to return to their essential elements to the food chain
fungi
what is mycology
the study of fungi
main role of fungi
to break down nonliving organic materials for them to return to their essential elements to the food chain
a fungi that is not human pathogenic does not attack living tissues or cause disease in humans
saprophytic
a common term for filamentous fungi, they are fast growing and capable of producing hundreds of thousands of spores in a short period of time
mold
Mold spores germinate and rapidly reproduce under what conditions?
in suitable moistures, temperature and nutrient conditions
mold spores disperse through?
air turbulence, water drops or insect activities
molds have been associated with a number of adverse health effects such as:
allergies, irritations, infections and toxic reactions.
is molds are aerobes or anaerobes?
aerobes
coz they require oxygen to grow
a hyphae that contains a cross-wall
septate hyphae
a hyphae that does not contain a cross-wall
aspetate hyphae
is hyaline hyphae colorless or no?
colorless
distinct color of dematiacious hyphae
distinct yellow to brownish color
formed on structure that are derived from aerial hyphae but are open to the air
conidiospores
is sporangiospored an asexual mold spores or no?
yes
a hyphae that is only associated with zygomycetes
sporangiospores
____ are formed enclosed within structures called sporangia that are borne on ____ that arise from aerial hyphae; these are associated only with zygomycetes
sporagiospores
three types of asexual spore are formed directly from hyphae
biatospores by budding
arthrospores by fermentation of hyphal tube
chlamydospores by complete wailing of nucleus within a hyphal segment
materials needed for mycology
fungi colony growth (SDA plate or rotten vegetables)
inoculating loop
Lactophenol cotton blue stain
Glass slides
Cover slips
Microscope
Procedure:
collect specimen from the fungus colony using an inoculating loop and smear onto the glass slide
put a drop of lactophenol cotton blue stain on the smear
place a coverslip on the stained smear and gently press
examine under microscope for the presence of fungi
aspergillus fumigatus
aspergillus fumigatus
microscopic examinations of these preparations usually involve the ____ and the ____ spores
hyphae
asexual spores
objective of this study
perform direct examination methods used in the study of fungi
isolate keratinophilic fungi through the hair baiting method
isolate and visualize keratinophilic fungi from skin of feet
what is the meaning of SDA
Sabouraud Dextrose Agar
room temp for a fungi to grow
25c
room temp for a fungi to grow
25c
