11. Laboratory techniques in mycology

Question 1

essential components of ecosystem and are widely distributed

Answer 1

fungi

Question 2

they are a large group eukaryotic organisms whose main role in nature is to break down nonliving organic materials in order to return to their essential elements to the food chain

Answer 2

fungi

Question 3

what is mycology

Answer 3

the study of fungi

Question 4

main role of fungi

Answer 4

to break down nonliving organic materials for them to return to their essential elements to the food chain

Question 5

a fungi that is not human pathogenic does not attack living tissues or cause disease in humans

Answer 5

saprophytic

Question 6

a common term for filamentous fungi, they are fast growing and capable of producing hundreds of thousands of spores in a short period of time

Answer 6

mold

Question 7

Mold spores germinate and rapidly reproduce under what conditions?

Answer 7

in suitable moistures, temperature and nutrient conditions

Question 8

mold spores disperse through?

Answer 8

air turbulence, water drops or insect activities

Question 9

molds have been associated with a number of adverse health effects such as:

Answer 9

allergies, irritations, infections and toxic reactions.

Question 10

is molds are aerobes or anaerobes?

Answer 10

aerobes
coz they require oxygen to grow

Question 11

a hyphae that contains a cross-wall

Answer 11

septate hyphae

Question 12

a hyphae that does not contain a cross-wall

Answer 12

aspetate hyphae

Question 13

is hyaline hyphae colorless or no?

Answer 13

colorless

Question 14

distinct color of dematiacious hyphae

Answer 14

distinct yellow to brownish color

Question 15

formed on structure that are derived from aerial hyphae but are open to the air

Answer 15

conidiospores

Question 16

is sporangiospored an asexual mold spores or no?

Answer 16

yes

Question 17

a hyphae that is only associated with zygomycetes

Answer 17

sporangiospores

Question 18

____ are formed enclosed within structures called sporangia that are borne on ____ that arise from aerial hyphae; these are associated only with zygomycetes

Answer 18

sporagiospores

Question 19

three types of asexual spore are formed directly from hyphae

Answer 19

biatospores by budding
arthrospores by fermentation of hyphal tube
chlamydospores by complete wailing of nucleus within a hyphal segment

Question 20

materials needed for mycology

Answer 20

fungi colony growth (SDA plate or rotten vegetables)
inoculating loop
Lactophenol cotton blue stain
Glass slides
Cover slips
Microscope

Question 21

Procedure:

Answer 21

collect specimen from the fungus colony using an inoculating loop and smear onto the glass slide

put a drop of lactophenol cotton blue stain on the smear

place a coverslip on the stained smear and gently press

examine under microscope for the presence of fungi

Question 22

aspergillus fumigatus

Answer 22

aspergillus fumigatus

Question 23

microscopic examinations of these preparations usually involve the ____ and the ____ spores

Answer 23

hyphae
asexual spores

Question 24

objective of this study

Answer 24

perform direct examination methods used in the study of fungi

isolate keratinophilic fungi through the hair baiting method

isolate and visualize keratinophilic fungi from skin of feet

Question 25

what is the meaning of SDA

Answer 25

Sabouraud Dextrose Agar

Question 26

room temp for a fungi to grow

Answer 26

25c

Question 27

room temp for a fungi to grow

Answer 27

25c