13 - Microbial Spoilage and Public Health Concerns pt 2 Flashcards
several milk spoilage bacteria can rely on what processes for energy generation?
proteolysis and lipolysis
lipolysis is favorable under what conditions?
at 4C. bc psychrotrophic bacteria have a faster growth rate here and produce lipases.
proteolysis is favorable under what conditions?
7C
what is the more common carbon source for bacterial spoilage in milk?
lactose
what are the main source of nitrogen in milk?
casein
what are non-protein nitrogen sources in milk?
- urea
- peptides
- amino acids
what are the major inhibitors of microbial growth in milk?
lactoferrin and lactoperoxidase
what does lactoferrin do?
binds iron, making it a limiting factor.
why isn’t lactoferrin as effective in cow’s milk as it is in human’s?
cow’s milk has more citrate which binds lactoferrin
what does lactoperoxidase do?
catalyzes the oxidation of thiocyanate and produces hypothiocyanite, a highly reactive oxidant
what are some of the less major natural inhibitors in milk?
lysozyme, specific immunoglobulins, folate, and vit B12
what is the limiting substrate in the lactoperoxidase system?
H2O2
adding H2O2 to milk will (increase/decrease) effectiveness of the lactoperoxidase system
increase
what microorgs are inhibited by the lactoperoxidase system?
LAB, coliforms, several pathogenic bacteria
true or false: it’s important to refrigerate raw milk before it’s pasteurized
true
what is the predominant bacteria in raw milk?
Pseudomonas sp.
what kind of respiratory bacteria will dominate spoiled refrigerated milk?
obligate aerobes
describe what happens to bacteria numbers over time during milk spoilage
- enterobacteriaceae increase but are eventually overtaken by Pseudomonadaceae
- if there’s spores, it’s usually from Bacillus genus
what does psychrotrophic milk spoilage rely on?
proteolysis. lipolysis to a lesser extent
what is the bacteria most associated with milk spoilage?
Pseudomonas sp.