13) Hypersensitivity Flashcards
define hypersensitivity rxn
exaggerated/inappropriate immune response with damage to host
basic mechanism of TI HS rxn
IgE mediated
binds to masts and basos
histamine release
general sx of TI HS rxn
rhinitis
asthma
anaphylaxis
basic mechanism of TII HS rxn
IgG/IgM binds to cell and activates complement
immediate HS rxn (within minutes)
type I
cytotoxic HS rxn
type II
immune complex HS rxn
type III
TIII HS rxn timeframe
within hours
TIV HS rxn timeframe
1-2 days
cell mediated/delayed type HS rxn
type IV
basic mechanism of TIII HS rxn
IgG or IgM - Ag complexes accumulate in blood/tissues
granulocytes release damaging enzymes
basic mechanism of TIV HS rxn
T-cell cytokines activate macros, causing damage
3 phases of TI HS rxn
- sensitization
- activation
- effector response
first exposure to Ag, IgE attaches to masts/basos
sensitization stage (type I)
reaginic Ab
IgE
stimultes/suppresses IgE production
stimulates: IL-4
suppresses: IFN-𝛾
begins activation phase of TI HS rxn
2 forms
challenge/2nd exposure to Ag
cutaneous injection
IV/parenteral injection
wheal & flare rxn part of type —- HS rxn
I
wheal & flare peaks in —— mins
sx
10-15
erythema
edema
“hives”
required for Ig to cause degranulation
2 Fc receptors crosslinked by Ag
triggers of mast cell degran
- IgE
- C3a, C5a
- drugs
- lectins
- extreme heat & cold
control mechanism for mast cell degran (TI HS rxn activation phase)
- adenylate cyclase converts ATP to cAMP during degranulation
- sustained increase in intracellular cAMP slows or stops degranulation
2 types of mediators involved in TI HS rxn effector phase
- preformed mediators - stores in granules
- newly-synthesized mediators
main preformed mediator
histamine
storage of histamine
electrostatic interaction with heparin
histamine binding sites
H1 (smooth muscle, endothelials)
H2 (mucosal membranes)
effects of histamine
- smooth muscle constriction
- vascular permeability
- release of stomach acid
antihistamines block —–
H1
chemotactic factors involved in effector phase of TI HS rxn
- GM-CSF
- IL-5
- TNF-a
- ECF
- PAF
- IL-8
late indicator of IgE mediated rxn
eos
newly-synthesized mediators and source
- leukotrienes (arachidonic acid + lipoxygenase)
- prostaglandins/thromboxanes (arachidonic acid + cyclooxygenase)
- PAF (platelets)
cause prolonged constriction of smooth muscle even in presence of histamine
leukotrienes
cause bronchoconstriction and chemotactic for grans
prostaglandins
thromboxanes
induces plt aggregation and release of histamine from them
potent bronchoconstrictor and vasodilator
PAF
mediator that can cause shock-like sx
PAF
arachidonic acid released from…
membrane lipids
phase of TI HS rxn occurring after mast cell degranulation and activation
late phase rxn
mechanism of late phase rxn
- ECF and IL-5 recruits eos and neuts to site
- they are activated
- eos release PAF, MPB, ECP
- neuts phagocytize Ag-AB complexes and release PAF, leukotrienes
neurotoxin and helminthotoxin
eosinophil cationic protein
examples of TI HS rxn
- allergic rhinitis/hay fever
- food allergies
- atopic dermatitis
- asthma
- insect bite allergies
airborne allergen reacts with IgE-sensitized mast cells in nasal passages/conjunctiva
allergic rhinitis (TI)
food allergen reacts with IgE-sensitized mast cells in GI tract
food allergy (TI)
allergen contacts skin, activating masts and attracting eos
atopic dermatitis
red and pus-filled skin rash
atopic dermatitis
chronic obstruction of lower airways related to chemicals, pollen, dust, viral Ag
asthma
2 methods of TI HS rxn detection
- skin allergy test
- RAST (quantitative)
RAST
radioallergosorbent test
explain RAST principle
- allergen on bead/disc placed in pt serum
- IgE binds
- wash
- add anti-IgE conjgate radioactively labelled
- measure radioactivity
pharm interventions for TI HS rxn
- cromolyn sodium: prevents influx of Ca, degranulation
- corticosteroids: block arachidonic pathway
- antihistamine: block H1
- epinephrine: reverses effects of histamine (life-threatening rxn)
method of inducing tolerance to Ag by injecting pt with increasing doses over time
immunotherapy/hyposensitization
2 ways hyposensitization works
- induction of tolerance
- production of IgG that binds Ag before IgE can
allergy shots are better for Ags ———-
in circulation
(less IgG on mucosa)
complement fixation in TII HS rxn leads to —- or —–
lysis or opsonization
examples of Ab-mediated cellular dysfunction
autoAb bind to cell receptors
- myasthenia gravis (ACh receptor)
- Graves (TSH receptor)
examples of TII HS rxn
- trxn
- cold agglutinin
- HDFN
- goodpastures
- pemphigus vulgaris
- myasthenia gravis
- Graves
- drug-induced (chloramphenicol)
Arthus rxn
TIII HS rxn
increasingly severe sx at site of inoculation
first mild redness and swelling
after several exposures - hemorrhagic, necrosis
complement fixes to Ab-Ag complexes and releases anaphylatoxins
TIII HS rxn
examples of TIII HS rxn
- serum sickness
- infection-associated immune complex disease (Goodpasture, rheumatic fever)
- hypersensitivity pneumonitis (Farmer’s lung)
serum sickness
TIII HS rxn
injection of serum from another species
make Ab to foreign Ig
———- size complexes most harmful
small/medium
large complexes removed by phagocytes
rheumatic fever
TIII HS rxn
follows type A strep infection
cause of Farmer’s lung
inhaling actinomycetes spores in moldy hay
can cause Arthus type rxn in lungs
examples of TIV HS rxn
- contact sensitivity (metals, poison ivy)
- allograft rejection
- cutaneous basophil hypersensitivity
TIV HS reaction description
- takes days
- erythema, induration
- does not cause necrosis
- resolves slowly
- mainly macros, few neuts
—– suppresses TH2 and expands TH1, contributing to macrophage activation in TIV HS rxn
IL-12
giant cells made of clusters of epitheloids formed by macros accumulating in tissue
granuloma
TIV HS rxn
rejected graft tissue is invaded by…
Ag-specific lymphs and monos
skin injection of Ag
takes 24 hours
redness, no induration
may have link to parasites
cutaneous basophil hypersensitivity
example of cause of cutaneous basophil hypersensitivity
tick bites
activates macros to release inflammatory mediators
IFN-𝛾
↑ expression of adhesion molecules on blood vessels
TNF-β