1.3 - Carbohydrates - Disaccharides And Polypeptides Flashcards
Glucose joined to glucose forms what?
Maltose
Glucose joined to fructose forms what?
Sucrose
Glucose joined to galactose forms what?
Lactose
When the monosaccharides join what type of reaction is it?
A condensation reaction
What bond is formed between monosaccharides?
Glycosidic bond
What type of reaction is it when a disaccharide is broken down into its monosaccharides?
Hydrolysis
What is added that causes the breakdown in hydrolysis?
Water
Some disaccharides are what?
Reducing sugars
In order to detect a non - reducing sugar it must be what?
Hydrolysised into its monosaccharide components by hydrolysis
What is the process of detecting a reducing sugar?
- If the sample is not already in liquid form, it must first be ground up in water
- Do the Benedict’s test on it if it fails to change colour then:
- Add another 2cm^2 of the food sample to 2cm^2 of dilute hydrochloric acid in a test tube and place the test tube in a gently boiling water bath for 5 minutes
- Slowly add some hydrogen-carbonate solution to the test tube
- Re do the Benedict’s test on it if it goes from blue black to orange brown then a reducing sugar is present
Why do you add dilute hydrocloric acid to the food sample?
It will hydrolyse any disaccharide present into its monosaccharides
Why do you add sodium hydrogen-carbonate solution to the food sample
To neutralise the hydrochloric acid (Benedict’s reagent wont work in acidic conditions)
What are polysaccharides?
Polymers, formed by combining together many monosaccharides
Polysaccharide are very large molecules meaning they are?
Insoluble
What is good about polysaccharides being insoluble?
Makes then suitable for storage
Some polysaccharides, such as cellulose are not used for storage but for what?
Giving structural support for plants
What is the test for starch?
Iodine test
What is the method for the iodine test?
- Place 2cm^2 of the sample being tested into a test tube
- Add two drops of iodine solution and shake or stir
- If there is starch present the solution will turn from orange to blue black