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AH Biology > 1.1a & 1.1b > Flashcards

1.1a & 1.1b Flashcards

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1
Q

Name a hazard in the lab

A

toxic chemicals

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2
Q

Hazards can be presented in the lab by…

A

substances, organisms and equipment

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3
Q

What is risk?

A

the likelihood of harm arising from a hazard

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4
Q

What must be carried out for a hazard?

A

risk assessments, risk control, method control

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5
Q

What is dilution?

A

reducing the concentration of a solution

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6
Q

A serial dilution involves…

A

repeated dilutions from a stock solution

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7
Q

What is a linear solution?

A

linear solutions differ by equal intervals

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8
Q

What is a log solution?

A

log solutions differ by a factor of 10

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9
Q

How can colourimeters be used to find the concentration of coloured solutions?

A

by measuring the absorption of light

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10
Q

Colourimeters can be used to find the…

A

concentration of colourful compounds in solution by measuring the absorbance of light through a sample

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11
Q

What is transmission of light?

A

the % of light that passes through a sample

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12
Q

When determining the concentration of an unknown sample, the absorbance of a series of…

A

standards of known concentration are recorded using a colorimeter

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13
Q

The unknown can then be determined after a…

A

calibration graph is plotted

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14
Q

What are the two most common protein standards?

A

BSA and gamma globulin

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15
Q

To produce a standard curve, a dye should be added to…

A

known concentrations of protein solutions

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16
Q

The absorbance of each solution is then measured using a colorimeter, with a filter that is…

A

complementary to the solution eg has an opposite colour wavelength

17
Q

A graph can be plotted with c___ on the x-axis and ___ on the y-axis.

A

concentration, absorbance

18
Q

The x-axis always has the…

A

independent variable

19
Q

What is pH?

A

a logarithmic scale of Hydrogen ion concentration

20
Q

What is a pH buffer?

A

a solution whose concentration does not change when adding a small amount of acid or alkali

21
Q

Which two things must you do before using a colorimeter?

A
  1. select a filter, that is complementary to the solution
  2. calibrate the colorimeter with a blank solvent (whichever solvent was used when making the solutions) and select a filter that is complementary to the solution

AH Biology (2 decks)

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