1.1.4 detecting proteins using antibodies Flashcards

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1
Q

what are immunoassay techniques used for

A

to detect and identify specific proteins

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2
Q

what do immunoassay techniques use to work

A

stocks of antibodies with the same specifity

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3
Q

what are monoclonal antibodies

A

those with the same specifity

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4
Q

what is an antibody specific to the protein antigen linked to

A

a chemical label; often a reporter enzyme producing a colour change.

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5
Q

what types of reporter enzymes are used to link with specific antibodies

A

chemiluminescence
fluroescence
reporter enzyme

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6
Q

when is western blotting used

A

after SDS-PAGE electrophoresis

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7
Q

how does western blotting work

A

the separated proteins from the gel are transferred onto a solid medium
the proteins can be identified using specific antibodies that have reporter enzymes attached

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