11. Chronic Inflammation I Flashcards
GRANULOMATOUS INFLAMMATION
“Tissue aggregates of epithelioid macrophages as part of a ____ immune response”
Do not confuse “Granulomatous inflammation” with: • \_\_\_\_ • \_\_\_\_ Granuloma • \_\_\_\_ Granuloma
cell-mediated
chronic granulomatous disease (CGD)
pyogenic
periapical
Acute vs Chronic Inflammation
Acute Duration: \_\_\_\_ Etiology: \_\_\_\_ Sign: \_\_\_\_ Symptom: \_\_\_\_ Outcome: \_\_\_\_ Cells: \_\_\_\_ Biology: \_\_\_\_
Chronic Duration: \_\_\_\_ Etiology: \_\_\_\_ Sign: \_\_\_\_ Symptom: \_\_\_\_ Outcome: \_\_\_\_ Cells: \_\_\_\_ Biology: \_\_\_\_
if someone has pneumonia (acute) they will know due to systemic inflammations but with chronic there might not be at the beginning until tissue is ____, ex. Peridontal disease.
hour/days eliminated red/heat/swelling \+++ resolution/abscess/scar/fibrosis mast/PMN/mac vascular/exudative
weeks/months/yrs persists indurated/hard --+ destruction/fibrosis mac/lymp "round cells" proliferative
destroyed
Where do cells come from?
Initially macrophages involved in inflammatory reactions are derived from the same source as neutrophils - the ____. You have monocytes in the blood that once get into the tissue are ____ in inflammation. Tissue like skin, interstinal tract.
Other kind of macrophages that reside – ____ cells in liver, ____ macrophages in lung , ____ in the brain tissue. These are generated from the progenitor cells in the ____ and ____ liver.
Monocyte – looks similar to a band cell but it is much the nucleus is much ____ and stains ____ rather than blue.
When monocyte becomes macrophage it takes the shape on the right most image
bone marrow kupfer alveolar microglia yolk sac fetal
smaller
pink
Properties of neutrophils and Macrophages
Neutrophils Origin: \_\_\_\_ Lifespan in tissue: \_\_\_\_ Response to stimuli: \_\_\_\_ Degranulation: \_\_\_\_ ROS: \_\_\_\_ Cytokine production: \_\_\_\_ NET formation: \_\_\_\_
Macrophages Origin: \_\_\_\_ Lifespan in tissue: \_\_\_\_ Response to stimuli: \_\_\_\_ Degranulation: \_\_\_\_ ROS: \_\_\_\_ Cytokine production: \_\_\_\_ NET formation: \_\_\_\_
• M1 clascially activated macrophages (\_\_\_\_) M2 (TGFbeta firboris) (\_\_\_\_)
HSCs in bone marrow 1-2 hour rapid, short-lived major response rapidly induced (respiratory burst) low levels or none prominent
HSCs in bone marrow (inflam); SC in yolk/fetal liver (resident)
inflam (day/weeks); resident (years)
slow, prolonged, transcription/cytokine
not prominent
not prominent
Major: M1: TNF, IL-1, IL-12, IL-6, chemokines; M2: IL-10, TGFbeta
No
pro-inflam
anti-inflam
• Macrophaegs work with T cells
○ Macrophage digest particles, but limited comapred to neutrophils
○ Express on ____, activate T cells
§ Th1: cytokine is ____; target = macrophage, happens when ____ pathogen, autoimmunity/crhornic
§ TH2: allergy > ____, ____, ____; target = eosinphils, against helminths and allergy
§ TH17: produce ____ and ____; activate neutrophils, extracell immunity
IFNgamma
IL4, IL5, IL13
IL17, IL12
M1 and M2 Macrophages • M1 – Activated by endotoxin, \_\_\_\_, and \_\_\_\_ matters – Produce \_\_\_\_, lysosomal enzymes and cytokines that induce \_\_\_\_
• M2 – Activated by cytokines other than \_\_\_\_ – NOT \_\_\_\_ – Promote \_\_\_\_, collagen synthesis \_\_\_\_ activation of M1 and M2?
You can go back to previous lecture, for most part the classically activated
macrophages are the ones that are activated by microbial product and ____ and IFNy1 which is produced from ____ cells and they produce ROS, enzymes and will phagocytose bacteria but it is not a prominent function, its really the neutrophils that do
that. They can also produce ____, TNF, ____, IL-6, chemokines that produce
inflammation.
The alternatively activated macrophages are induced by a different set of cytokines they produce IL1-beta which are ____ and ____ which lays down collagen.
These macrophages are activated in repair and fibrosis and they are antiinflammatory.
It is possible to they are activated sequentially, M1 then M2.
Not everyone believe they exist but most people believe there are two types that have
different function
IGNgamma
particulate
ROS
inflammation
IFNgamma
microbicidal
angiogenesis
sequential
TLR1
Th1
IL1
IL12
antiinflammatory
TGFb
Acute vs Chronic Inflammation
• Acute ○ Congested BV, neutrophils, redness • Chrnic ○ Foci of \_\_\_\_ ○ Normal lung parenchyma replaced by \_\_\_\_ ○ \_\_\_\_ epithelial that’s normally not there
inflam cells
fibrotic tissue
cuboidal
Retrograde Infection
an infection that spreads along a tubule or duct against the ____
of secretions.
* Tissue destruction * Blocked \_\_\_\_… infection that is opposite to salivary flow
flow
salivary gland
Chronic Sialadentitis
• Inflammation of \_\_\_\_ • Foci of inlam cells ○ Not \_\_\_\_, but macros and lympho's • Normal glandular cells replaced by \_\_\_\_ and \_\_\_\_
salivary gland
neutrophils
foci
fibrosis
Persistence of Etiologic Agents
• Macrophages unable to kill bacteria:
–____ and ____ (→DTH)
• Macrophages unable to degrade bacteria:
– Certain strains of ____
- Viruses and fungi:
- T cell/macrophage-mediated (____)
• Caused by particulate or physical agents:
– Acid, ____, talc, asbestos, ____, gall stones
• Immunecomplexes:
– In blood vessels, ____, kidneys
• Uric acid = gout ○ \_\_\_\_, macro's cannot digest these • Immune complexes deposits…
tuberculosis leprosy streptococci DTH silica uric acid joints inorganic
Persistence of etiologic agents in Periodontal disease • Burst model: – Tissue destruction occurs in \_\_\_\_ followed by \_\_\_\_ periods. • Slow continuous model: • Homeostatic break down?
Persistence of etiologic agent in periodontal disease its been known that it is not a
continuous destruction in the bone. People with disease have bone destruction for a
period of time then its ____, then ____ up again.
Different models on to why it happens
The best model proposed was talked about with Dr. Hasengalis. Normally gingival
crevis has 100,000 types of bacteria that are commensal and symbiotic and happy and
good for you, but in susceptible individuals or during periodontal disease there is immune regulatory defect or systemic disease or other factors that contribute to
periodontal disease or you might have infection with ____ (ex, P.
gingivalis). There is a breakdown of ____, and the normal commensal bacteria
become ____ and involved in periodontal disease.
bursts
quiescent
quiet
flares
keystone pathogen
homeostasis
dysbiotic
• Perio is caused by ____ and bacteria in bioifilm
• Inflamed gingiva > periodontal bone loss (takes long)
• Current model:
○ Symbiotic microbitota > dysbitoic due to ____ > recruit neuetrophils in gingival crevice
○ Neutrophils try to protect (acute lasts for few days), but here, chronic, the neutrophils won’t be protective bc the etiology are persisent
○ Macro’s/T cells activated, produce cytokines (TNFalpha, IL1, IL17) which induce activation of ____
○ IL17 recruits ____, which also come in, and produce ROS and ____, and play a role in gingival tissue degradation
• NEUTROPHILS CONTRIBUTE TO ____
• LAD > HOW DO YOU GET PERIO DISEASE
○ Neutrophils are in BV but cannot get out, and cannot be effero, and providing signal for IL23 > producing IL17 causing bone loss
• Both LAD and perio it is due to infection
keystone pathogen osteoclasts neutrophils proteases periodontal bone loss
Rheumatoid Arthritis
Autoimmune (____ immune system, T and B cells activated), and mistakes part of body is ____
With Rheumatoid Arthritis we don’t know if the antigen is a microbe or self component.
Whatever it is, it would be processed by macrophage and presented to T cell in context of MHC.
The T cells produce cytokines which activate B cell and macrophages and
leads to the formation of R-factor which leads to ____ formation which
deposits within the ____ and eventually bring in neutrophils.
Also cytokines produced activate Fibroblasts and chondrocyte to promote their
proliferation. Cytokines also activate endothelial cells and when endothelial cells are
activated they increase expression of ____ molecules to help bring in leukocytes.
The result is the formation of ____, bone destruction, fibrosis and ____.
In the figure you can see the normal joint. There is synovial fluid in-between the joint,
which is important for the joint to function properly. In rheumatoid arthritis, you get
neutrophil recruitment because of immune complexes, because of activated B cells you
get antibodies, macrophages are activated, dendritic cells are there, and lymphocytes
are there. All these chronic inflammation cells are there and you get the formation of
pannus. (Missing from this slide): Pannus consist of ____ cells, ____ tissue,
____ connective tissue. It under goes fibrosis ankylosis, and instead of fluid between
the joints you have ____ tissue bridging the two parts of the joint.
It can also undergo ____, and you can have bony ankylosis, related to more
of a severe arthitis.
adaptive
foreign
immune complex
joints
adhesion
pannus
ankylosis
inflammatory
granulation
fibrous
fibrous conenctive
ossification
- Acute Abscess
- Chronic Abscess
- Acute: ____ material only
- Chronic: ____ tissue that is encapsulating ____ exudate; and when heals it leads to scar and fibrosis
- Acute, chronic and granulation can go on at same ____
purulent
granulation
purulent
time
Localized chronic duodenal ulcer
Another example is localized duodenum stomach ulcer. In the stomach, there are
____ forces that are trying to damage mucosa, ex. gastric acidity and peptic
enzymes, but you also, have ____ forces and whether you have homeostatic
disease matters which factor is stronger.
Defensive forces include, surface ____,
____ secretion, and regenerative property of ____ cell, and elaboration of
____ which help to maintain homeostasis.
This hemostasis can breakdown by impaired defense mechanism, or increase damage.
Examples of both are listed on the slide.
Chronic peptic acid - if you have more acid than normal, than it will act as a irritant that
damage epithelial cells and you will get an acute ____.
Non specific acute inflammation will try to wall off acid, but if the etiology persist for a long time, the acute inflammation
will lead to the formation of ____ tissue and eventually ____. As long as the
acid persist you will have damage (____), ____ inflammation, ____ tissue formation and ____, a dynamic interaction between each factor.
damaging
defensive
mucosa
bicarb
epithelial
prostaglandins
ulcer
granulation fibrosis ulcer acute granulation fibrosis
