1.1 Basic Hematology Concepts and Laboratory Procedures Flashcards
Insufficient centrifugation will result in:
A. A false increase in hematocrit (Hct) value
B. A false decrease in Hct value
C. No effect on Hct value
D. All of these options, depending on the patient
A. A false increase in hematocrit (Hct) value
Variation in red blood cell (RBC) size observed on the peripheral blood smear is described as:
A. Anisocytosis
B. Hypochromia
C. Poikilocytosis
D. Pleocytosis
A. Anisocytosis
Which of the following is the preferred site for bone marrow aspiration and biopsy in an adult?
A. Iliac crest
B. Sternum
C. Tibia
D. Spinous processes of a vertebra
A. Iliac crest
Mean cell volume (MCV) is calculated by using the following formula:
A. (Hgb ÷ RBC) × 10 where Hgb is hemoglobin in g/dL
B. (Hct ÷ RBC) × 10
C. (Hct ÷ Hgb) × 100
D. (Hgb ÷ RBC) × 100
B. (Hct ÷ RBC) × 10
What term describes the change in shape of erythrocytes seen on a Wright-stained peripheral blood smear?
A. Poikilocytosis
B. Anisocytosis
C. Hypochromia
D. Polychromasia
A. Poikilocytosis
Calculate the mean cell hemoglobin concentration (MCHC) by using the following values:
Hgb: 15 g/dL (150 g/L) Hct: 47 mL/dL (0.47)
RBC: 4.50 × 106/μL (4.50 × 1012/L)
A. 9.5% (0.095)
B. 10.4% (0.104)
C. 31.9% (0.319)
D. 33.3% (0.333)
C. 31.9% (0.319)
A manual white blood cell (WBC) count was performed. In total 36 cells were counted in all 9-mm2 squares of a Neubauer-ruled hemacytometer. A 1:10 dilution was used. What is the WBC count?
A. 0.4 × 109/L
B. 2.5 × 109/L
C. 4.0 × 109/L
D. 8.0 × 109/L
A. 0.4 × 109/L
When an erythrocyte containing iron granules is stained with Prussian blue, the cell is called a:
A. Spherocyte
B. Leptocyte
C. Schistocyte
D. Siderocyte
D. Siderocyte
A 7.0-mL ethylenediaminetetraacetic acid (EDTA) tube is received in the laboratory containing only 2.0 mL of blood. If the laboratory is using manual techniques, which of the following tests will most likely be erroneous?
A. RBC count
B. Hgb
C. Hct
D. WBC count
C. Hct
A 1:200 dilution of a patient’s sample was made, and 336 RBCs were counted in an area of 0.2 mm2. What is the RBC count?
A. 1.68 × 1012/L
B. 3.36 × 1012/L
C. 4.47 × 1012/L
D. 6.66 × 1012/L
B. 3.36 × 1012/L
What phagocytic cells produce lysozymes that are bacteriocidal?
A. Eosinophils
B. Lymphocytes
C. Platelets (PLTs)
D. Neutrophils
D. Neutrophils
If a patient has a reticulocyte count of 7% and Hct of 20%, what is the corrected reticulocyte count?
A. 1.4%
B. 3.1%
C. 3.5%
D. 14%
B. 3.1%
A decreased osmotic fragility test would be associated with which of the following conditions?
A. Sickle cell anemia
B. Hereditary spherocytosis (HS)
C. Hemolytic disease of the fetus and newborn
D. Acquired hemolytic anemia
A. Sickle cell anemia
What effect would using a buffer at pH 6.0 have on a Wright-stained smear?
A. RBCs would be stained too pink
B. WBC cytoplasm would be stained too blue
C. RBCs would be stained too blue
D. RBCs would lyse on the slide
A. RBCs would be stained too pink
Which of the following erythrocyte inclusions can be visualized with supravital stain but cannot be detected on a Wright-stained blood smear?
A. Basophilic stippling
B. Heinz bodies
C. Howell–Jolly bodies
D. Siderotic granules
B. Heinz bodies
A falsely elevated Hct is obtained. Which of the following calculated values will not be affected?
A. MCV
B. Mean corpuscular hemoglobin (MCH)
C. MCHC
D. Red blood cell distribution width (RDW)
B. Mean corpuscular hemoglobin (MCH)
A Miller disk is an ocular device used to facilitate counting of:
A. PLT
B. Reticulocytes
C. Sickle cells
D. Nucleated red blood cells (NRBCs)
B. Reticulocytes
SITUATION: RBC indices obtained on an anemic patient are as follows: MCV 88 μm3 (fL); MCH 30 pg; MCHC 34% (0.340). The RBCs on the peripheral blood smear would appear:
A. Microcytic, hypochromic
B. Microcytic, normochromic
C. Normocytic, normochromic
D. Normocytic, hypochromic
C. Normocytic, normochromic
All of the following factors may influence the erythrocyte sedimentation rate (ESR) except:
A. Blood drawn into a sodium citrate tube
B. Anisocytosis, poikilocytosis
C. Plasma proteins
D. Caliber of the tube
A. Blood drawn into a sodium citrate tube
What staining method is used most frequently to stain and manually count reticulocytes?
A. Immunofluorescence
B. Supravital staining
C. Romanowsky staining
D. Cytochemical staining
B. Supravital staining
The Coulter principle for counting of cells is based on the fact that:
A. Isotonic solutions conduct electricity better than cells do
B. Conductivity varies proportionally to the number of cells
C. Cells conduct electricity better than saline does
D. Isotonic solutions cannot conduct electricity
A. Isotonic solutions conduct electricity better than cells do
A correction is necessary for WBC counts when NRBCs are seen on the peripheral blood smear because:
A. The WBC count would be falsely lower
B. The RBC count is too low
C. NRBCs are counted as leukocytes
D. NRBCs are confused with giant PLTs
C. NRBCs are counted as leukocytes
Using an electronic cell counter analyzer, an increased RDW should correlate with:
A. Spherocytosis
B. Anisocytosis
C. Leukocytosis
D. Presence of NRBCs
B. Anisocytosis
Given the following values, which set of RBC indices suggests spherocytosis?
A. MCV 76 μm3 MCH 19.9 pg MCHC 28.5%
B. MCV 90 μm3 MCH 30.5 pg MCHC 32.5%
C. MCV 80 μm3 MCH 36.5 pg MCHC 39.0%
D. MCV 81 μm3 MCH 29.0 pg MCHC 34.8%
C. MCV 80 μm3 MCH 36.5 pg MCHC 39.0%
Which of the following statistical terms reflects the best index of precision when comparing two complete blood count (CBC) parameters?
A. Mean
B. Median
C. Coefficient of variation
D. Standard deviation
C. Coefficient of variation
Which of the following is considered a normal Hgb?
A. Carboxyhemoglobin
B. Methemoglobin
C. Sulfhemoglobin
D. Deoxyhemoglobin
D. Deoxyhemoglobin
Which condition will shift the oxyhemoglobin dissociation curve to the right?
A. Acidosis
B. Alkalosis
C. Multiple blood transfusions
D. Increased quantities of Hgb S or C
A. Acidosis
What is the major type of leukocyte seen in the peripheral blood smear from a patient with aplastic anemia?
A. Segmented neutrophil
B. Lymphocyte
C. Monocyte
D. Eosinophil
B. Lymphocyte
What is the normal WBC differential lymphocyte percentage (range) in the adult
population?
A. 5%–10%
B. 10%–20%
C. 20%–44%
D. 50%–70%
C. 20%–44%
In which age group would 60% lymphocytes be a normal finding?
A. 6 months–2 years
B. 4–6 years
C. 11–15 years
D. 40–60 years
A. 6 months–2 years
Which of the following results on an automated differential suggests that a peripheral blood smear should be reviewed manually?
A. Segs = 70%
B. Band = 6%
C. Mono = 15%
D. Eos = 2%
C. Mono = 15%
Which is the first stage of erythrocytic maturation in which the cytoplasm is pink because of the formation of Hgb?
A. Reticulocyte
B. Pronormoblast
C. Basophilic normoblast
D. Polychromatic normoblast
D. Polychromatic normoblast
Which of the following can shift the Hgb oxygen (O2) dissociation curve to the right?
A. Increases in 2,3 DPG
B. Acidosis
C. Hypoxia
D. All of these options
D. All of these options
Which of the following Hgb configurations is characteristic of Hgb H?
A. γ4
B. α2-γ2
C. β4
D. α2-β2
C. β4
Autoagglutination of RBCs at room temperature can cause which of the following abnormal test results?
A. Low RBC count
B. High MCV
C. Low Hct
D. All of these options
D. All of these options
