1d- Detecting proteins using antibodies Flashcards

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1
Q

Immunoassay techniques use antibodies with the same what?

A

Specificity

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2
Q

What are immunoassay techniques used to detect and identify?

A

Specific proteins

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3
Q

What are monoclonal antibodies?

A

Stocks of antibodies with the same specificity

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4
Q

An antibody specific to a protein antigen is linked to what and why?

A

A chemical label, the label is often a reporter enzyme producing a colour change. This label allows scientists to detect when binding occurs

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5
Q

What are some examples of reporter enzymes which can be used as chemical labels?

A
  • chemiluminescence

- fluorescence

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6
Q

What is western blotting?

A

A technique used after SDS-PAGE electrophoresis

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7
Q

What happens during western blotting?

A

The separated proteins from the gel are transferred (blotted) onto a solid medium, The proteins can then be identified using specific antibodies that have reporter enzymes attached

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