10.1 Escherichia Coli Flashcards
Classification of Escherichia coli
Gram negative cocci bacteria (red stain)
Energy source of Escherichia coli and its waste product
Sugar lactose
Waste product: lactic acid
Serology of Escherichia coli
Using antibodies to detect different bacterial surface antigens (O,H,K,F antigens)
Role of E.coli in health and disease
What can be used for growing E.coli
MacConkey agar
-grows into pink/red colonies if the pH becomes acidic (when lactose➡️lactic acid)
What are the 6 strains of diarrhoeagenic E.coli
Enterotoxigenic E.coli (ETEC)
Enteropathogenic E.coli (EPEC)
Shiga toxin-producing E.coli (STEC)
Enteroaggreagative E.coli (EAEC)
Enteroinvasive E.coli (EIEC)
Diffusers adherent E.coli (DAEC)
Which strains of diarrhoeagenic E.coli are the most common in young children in the developing world ?
EPEC (Enteropathogenic E.coli)
EIEC (Enteroinvasive E.coli)
Common conditions caused by E.coli
UTI
Intestinal infections (e.g. travel related diarrhoea)
Sepsis
Neonatal meningitis
Management of UTI-related E-coli infection
Antibiotics:
Trimethoprim
Nitrofuratoin
Prevention of diarrhoea-related E.coli infection
Avoid food&drink that could be contaminated with bacteria
-raw fruits, vegetables
-raw seafood, undercooked meat poultry
-unpasteurised dairy products
-untreated water
Treatment of diarrhoea-related E.coli infection
-Clear liquids to prevent dehydration&loss of electrolytes
-Oral rehydration solutions
-Avoid antibiotics
What are the two toxins produce by ETEC
Heat-stable toxin
Heat-labile toxin
How can Enterotoxigenic E. coli cause diarrhoea
-toxins stimulating the lining of the intestines
-causing them to secrete excessive fluid
-producing profuse watery diarrhoea & abdominal cramping
What does Shiga toxin-producing E. coli (STEC) cause
Haemorrhagic colitis
Haemolytic uraemia syndrome
Describe how E. coli causes UTI
-Uropathogenic E. coli transfer from the rectum to the urethra and then migrate to the bladder
-Causes cystitis
