1) f) Aseptic Techniques

Question 1

Aseptic techniques eliminate?

Answer 1

Aseptic technique eliminates unwanted
microbial contaminants when culturing micro-organisms or cells

Question 2

What does aseptic techniques involve?

Answer 2

Aseptic technique involves
* the sterilisation of equipment
and culture media by heat or chemical means
* subsequent exclusion of microbial contaminants.

Question 3

How can a microbial culture be started?

Answer 3

A microbial culture can be started using an:
inoculum of microbial cells on an agar
medium
or in a broth with suitable nutrients

Question 4

What medium are Animal Cells grown in?

Answer 4

Animal cells are grown in medium containing growth factors from serum

Question 5

What are growth factors?

Answer 5

Growth factors are proteins that promote cell growth and proliferation.

Growth factors are essential for the culture of most animal cells.

Question 6

Describe what primary cell lines can do in culture?

Answer 6

In culture, primary cell lines can divide a
limited number of times

Question 7

Describe what tumour cell lines can do in culture?

Answer 7

Tumour cells lines can perform unlimited divisions

Question 8

What is serial dilution used to achieve?

Answer 8

Serial dilution is often needed to achieve a suitable colony count

Question 9

What is vital staining used for?

Answer 9

Vital staining is required to identify and count viable cells
Vital Cells only stain DEAD CELLS!

Question 10

Plating out of a liquid microbial culture on solid media allows for?

Answer 10

Plating out of a liquid microbial culture on solid media allows the
number of colony forming units to be counted
and
the density of cells in the culture estimated

Question 11

Many ________ ________ ______ that promote the _______ of ________ _________ of _______ and _________>

Answer 11

Many culture media exist that promote the growth of specific types of cells and
microbes.