1. Estrous Cycle of the Bitch Flashcards

1
Q

do bitches ovulate mature eggs? how long do eggs take to become mature? does the oocyte need to be mature to be fertilized?

A

Do not ovulate mature eggs
◦ Takes 48 hours on average to become mature
◦ Canine oocytes have longer lifespan > extends the breeding period but fertilization can only occur once the oocytes are mature.

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2
Q

what hormone level rises before ovulation?

A

Progesterone levels begin to rise prior to ovulation
◦ Use progesterone to determine when ovulation occurs
◦ Hormones are the same pregnant or not

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3
Q

The stages of the canine estrous cycle

A

proestrus, estrus, diestrus, anestrus

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4
Q

are canines poly or mono estrus?

A

monoestrus, non seasonal

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5
Q

proestrus+ estrus =

A

heat

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6
Q

when is bitch puberty?

A

When the first proestrus occurs
Occurs earlier in small breeds vs. large breeds
◦ Average 8-14 months (range 6 – 26 months)

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7
Q

should we breed on first heat?

A

no

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8
Q

how is the first heat likely to differ from subsequent heats?

A

Likely to be “irregular” compared to other cycles ◦ Split or silent heats common

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9
Q

what is a split heat?

A

Split heat = heat starts then stops without ovulation. Starts again later.

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10
Q

what is a silent heat?

A

Silent heat = very little vaginal discharge.

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11
Q

4 strategies we can use to determine the stage of estrus

A

Vaginoscopy
Vaginal cytology
Progesterone levels
Behavior/receptivity

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12
Q

average length of proestrus?

A

Average length = 9 days (0-27 day range)

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13
Q

what day of heat do we see the first drop of blood?

A

day 1

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14
Q

what hormone level rises during proestrus? how does this effect the vagina and vulva?

A

Estrogen levels are rising
◦ Edema of vaginal epithelium; edema of the vulva

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15
Q

external cues of proestrus?

A

◦ Swollen vulva
◦ Serosanguineous vaginal discharge
◦ May start “flagging”

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16
Q

behavioural changes during proestrus?

A

Males are interested in the bitch
*Bitches are typically not receptive yet
*Bitches may mount other bitches
*Bitches in heat may become aggressive towards other bitches

17
Q

vaginal epithelium changes during proestrus?

A

Vaginal epithelium > goes from 5-10 cell layers to 10-20 cell layers
Cytology of vaginal swab
*Parabasal cells, intermediate cells

18
Q

average length of estrus

A

Average length = 9 days (4-24 day range)

19
Q

hormone changes during estrus

A

Stage at which LH surge occurs, ovulation occurs and fertile period occurs = when breeding occurs “standing heat”

Estrogen levels elevated initially; peak; starts decreasing at LH

Progesterone levels start to rise at LH, continue to elevate at ovulation & remain elevated

20
Q

how can we estimate the LH surge?

A

We can use progesterone to estimate the LH surge
◦ Coincides with the initial progesterone rise

Or can use in house LH test (Witness® LH)
◦ Usually reserved for important breedings; frozen semen breedings
◦ Can get costly, time consuming
◦ More accurate

21
Q

how does LH surge timeline relate to ovulation?

A

LH surge occurs 48 hours before ovulation

22
Q

how do we measure progesterone?

A

Ran on SERUM (ideally separated 20-30 mins after blood draw)
Place in red top tube
◦ No serum separator tubes

23
Q

vulva and vagina changes during estrus

A

Vulva becomes less turgid as estrogen decreases, serosanguineous discharge can diminish, can become lighter
Edema of vaginal epithelium decreases = crenulation

24
Q

what behaviours will the female engage in during her fertile period?

A

Female should be receptive to male during fertile period ◦ Flagging
◦ Seeks out the male

25
Q

vaginal epithelium characteristics during estrus?

A

Vaginal epithelium > 20-30 cell layers at estrogen level peak
Cytology of vaginal swab
◦ Cornified epithelial cells

26
Q

length of diestrus?

A

Length = average 2 months (range 56-58 days if pregnant; if not pregnant 56-90 days)
◦ If pregnantà62-64 days from ovulation (canine gestation = 63 days +/- 1 day)

27
Q

is bitch receptive to breeding during diestrus?

A

Bitch is no longer in “heat”
◦ No longer receptive to breeding

28
Q

when does diestrus occur relative to ovulation?

A

Occurs 6 days after ovulation (or 8 days from the LH surge)

29
Q

vulva and vagina changes during diestrus?

A

Vulva diminishes in size, vaginal discharge subsides, vaginal epithelium flattens

30
Q

hormone profile during diestrus?

A

Period of time when progesterone is high; estrogen is low

31
Q

vaginal epithelium characteristics during diestrus?

A

Vaginal epithelium peels off > goes back to 5-10 cell layers > 2-4 cell layers

Cytology of vaginal swab
◦ Parabasal cells
◦ Intermediate cells
◦ Neutrophils

32
Q

length of anestrus?

A

Length = average 4 months (range 4-12 months)

33
Q

what hormone drops during anestrus?

A

Progesterone drops

34
Q

what is anestrus? what is it important for? vaginal and vulvar character?

A

Period of reproductive quiescence Important stage for uterine involution
◦ Uterine involution is a necessary process Bitches are not receptive to breeding
Vulva small, no vaginal discharge

35
Q

Reproductive Consultation - why is it important?

A

◦ Our responsibility to educate clients on responsible breeding
◦> Time commitment
◦> $$$
◦ Should only be breeding mature, healthy, good dogs
◦> Clearances
◦>Representative of the breed

36
Q

Bitch breeding management considerations

A

History
◦ Medical
◦ Reproductive (Brucella canis status)

Physical exam

Reproductive exam
◦ Digital palpation
◦ Vaginal swab for cytology ◦ Vaginoscopy

Serum progesterone levels
◦ Usually blood taken every 2 days

37
Q

Breeding methods

A

Natural

Vaginal artificial insemination (AI)
◦ Plastic pipette ($)
◦ MAVIC catheter ($$)

Transcervical insemination (TCAI)
◦ $$$
◦ Semen is directly deposited in uterus ◦ Can usually do without any sedation

Surgical insemination (SAI)
◦ $$$$
◦ Semen is directly deposited in the uterus
◦ More invasive than TCAI – surgery under general
anesthesia

Pregnancy rates equivalent for TCAI as they are for SAI

38
Q

Types of semen

A

Fresh
◦ Can breed with standard vaginal AI, MAVIC catheter or TCAI

Fresh chilled
◦ Semen collected, evaluated (hopefully), processed elsewhere & shipped
◦ Is stored cool, not frozen
◦ Increased timing is required > need to give stud dog owner 24-48hrs notice
◦ Should be shipped 24hr priority > allows for semen from the US or in other parts of Canada to be used
◦ Standard vaginal AI, MAVIC or TCAI

Frozen
◦ TCAI or SAI only
◦ More in depth timing is required (confirm ovulation date with LH witness test)
◦ Progesterone timing every 2 days
◦ Stored in liquid nitrogen
◦ Can be shipped internationally (Europe, Australia, etc.).

39
Q

Take home points: estrogen levels during heat and effect, pogesterone surge timiing, LH surge timing, fertile period. More processed semen means what?

A

Estrogen is elevated in heat
◦ Edema of vulva
◦ Edematous vaginal epithelium
◦ Cornification on cytology

Progesterone rises prior to ovulation
◦ Use progesterone to determine ovulation date
◦ Rise typically coincides with LH surge

LH surge occurs 48 hours prior to ovulation Dogs ovulate immature eggs
◦ Fertile period = 2-4 days post ovulation

The more ”processed” the semen is = more precise timing required