02.01 Histology of the Cell Flashcards
What are the steps of Slide Preparation?
- Fixation - Termination of Cell Cycle, Prevention of Autolysis and Preservation of parts by using Fixative
- Dehydration - Removal of water using ethyl alcohol
- Clearing - Removing alcohols and lipids by using lipid solvent making it transparent
- Embedding - Specimen baked using paraffin making it rigid for sectioning
- Sectioning - Done Using Microtome
- Staining - Using the proper stain to identify specific parts
Mnemonic: Fajardo Do Crunches, Eleven Sit-ups, and Suicide
Hematoxylin and Eosin.
Hematoxylin: Basic, Basophilic (Reacts with Acids), Blue
Eosin: Acidic, Acidophilic (Reacts with Base), Pink
Identifies: Nucleus, Cytoplasm and Collagen
Periodic Acid Schiff
Glycogen and Carbohydrate rich structures such as the Liver
Magenta
Mnemonic: Please PASs the SUGAR MAGENTA.
Wright and Giemsa.
Blood cells
Pink: Erythrocytes and Eosinophil Granules
Purple: Leukocytes and Basophilic Granules
Blue: Cytoplasm of Monocytes and Lymphocytes
Mnemonic:
PEE
PLeBa
BCLy
Orcein Elastic Stain
Walls of Artery, and parts with Elastic fibers
Brown
Mnemonic: OBrF!
Silver Stain
Reticular Fiber (Liver) Black
Mnemonic: SiR F! Black!
Iron Hematoxylin Stain
Muscle Striations and Intercalated Disks
Black
Mnemonic: I aM IB
Masson’s Trichrome Stain
For muscles, collagens, fibrin and erythrocytes (based on porosity)
LB: Collagen
DB: Nucleus
Red: Muscles and Cytoplasm
Mnemonic: MM C FE! LaBo Co. Di Ba 'Nay? Right, Mother Cy?
What are the functions of Glycocalyx?
Cell Recognition, Intercellular Adhesion, Mechanical and Chemical Protection.
Mnemonic: C! IM Chiba loves Glycocalyx!
Organization of Eukaryotic Chromosome.
- DNA Structure
- Nucleosome
- Solenoid Structure
- Extended Chromatin
- Condensed Chromatin
- Condensed Chromosome `
Nuclear Pore Complex
8 particles (inner and outer) 8 radially arranged spokes
Principal Barrier to movements of particles >10nm
RER vs SER
RER: Proteins secreted and Plasma Membrane
SER: Fatty Acids Lipids Synthesis, Detox and Metab
What are the roles of the Golgi Apparatus?
Packaging, Concentration Modification, Sorting, Membrane Synthesis and Recycling
Mnemonic: GAP Sa MC!
What is the principal biochemical activity happening in the mitochondria?
Oxidative Phosphorylation
Endosymbiotic Theory
Mitochondria coming from free living bacterial ancestors. Hence Self-sufficient.
Peroxisomes
Involved in detox of alcohols, toxic drugs, and Uric acid
Mnemonic: PERO DAT Urine!
What are the different types of Inclusion?
Glycogen, Lipids, Protein Pigment
Mnemonic: Included Guests Lang Po
Microtubules
Tracks, Elongation, Maintenance of cell shape, Beating of structures and Movement
Mnemonic: MICROTUBULES TEMBa Mo!
