017 CAL: skeletal muscle histology Flashcards
what is this an image of?
- skeletal muscle in iron haematoxylin stain, longitudinal section
what type of muscle is this and how do you know?
- skeletal
- striations
- nuclei on periphery
what type of muscle is this and what is I, H, A?
- skeletal
- I = light I band (actin)
- H = H zone = myosin only
- A = dark A band (myosin and actin overlap)
what is this an image of?
- skeletal muscle in transverse
- silver stain
- can see reticular fibres
what is A?
adipose tissue
what is B?
blood vessels
what are the red arrows pointing to?
- endomysium
- connective tissue around each individual muscle fibre
what is stained black in this image?
- reticular fibres
- in the endomysium, perimysium, epimysium, around blood vessels, around adipose tissue
what is this an image of?
- skeletal muscle H and E
- transverse and longitudinal section
- transverse = blobs
- longitudinal = long fibres
what is this an image of?
- skeletal muscle inserting into a tendon (at bottom in orange)
why are there so many nuclei between the junction of the muscle and tendon?
- fibroblast nuclei
what is this an image of?
- skeletal muscles and its motor end plates / neuromuscular junctions
- iron haematoxylin stain
what is NT?
nerve trunk
what is MEP?
motor end plate
what is B?
branch of axons
what is BV?
blood vessel
what is TE?
trailing end = thin end of axon = probably a muscle spindle
what is MS?
muscle spindle
what is MS?
muscle spindle
what is skM
skeletal muscle fibre
what is this an image of?
- skeletal and smooth muscle and fibrous connective tissue ( F = fibroblasts)
- in oesophagus
- H and E stain
describe the differences between Sk and Sm
-Sk = round oval nuclei at periphery of cell, very long cells, striated, some cells have multiple nuclei, some have none
- Sm = elongated nuclei, spindle shape, in centre of cell, each cell has 1 nucleus
how do you histologically differentiate between fibroblasts and smooth muscle nuclei?
- fibroblast nuclei are pointy and curved
- smooth muscle are more cigar-shaped in spindle shape cells
what type of muscle is this?
- smooth muscle in transverse
describe what is between the smooth muscle fibres/cells in this image
- septae of connective tissue (mostly type 1 collagen and fibroblasts)
- no equivalent endomysium = smooth muscle cells are in direct contact with each other via gap junctions
what is this an image of?
- skeletal and smooth muscle in transverse of the oesophagus
CP = capillary
what is this an image of?
- smooth muscle and myenteric ganglion of stomach
what is LM and CM?
- longitudinal muscle and circular muscle of the muscularis externa
what is MP?
myenteric plecus
what is GC?
glial cells in the ganglia of the myenteric plexus
what is N?
Nerve cells
what is Ct?
- connective tissue outlining the ganglion
what is the histological difference between nerve cells and glial cells?
- glial cells = smaller denser nuclei
- nerve cell bodies = large clear, a circular nucleus with a prominent nucleolus and lots of cytoplasms
what is this an image of?
- smooth muscle of the urinary bladder
what is the epithelium type in this image?
- transitional epithelium = urinary
