Yotal Protein Flashcards
Method is based on the reports of
__________ and __________ et al.
weischelbaum and gornal et al.
the simplest of such compounds formed by heating urea term as
Biuret reaction
The reagent used is
Copper sulfate pentahydrate
Color produce total protein
Violet
Wavelength: Measured at
550nm
Rgt storage & Stability: kit
2-8C
Rgt storage & Stability: reagent
2-30C
Rgt storage & Stability: standard
2-8C
Spx collection or sample used
Serum only
Why does protein don’t used plasma as sample
It can result increase
Interfering Substances of total protein
Gross hemolysis & lipemia.
Interfering substances that don’t used in total protein
Urine and spinal fluid (decreased protein
Stand cuvets for atleast
5 mins
Read total protein within
1 hour
Anticoagulant used of total protein
No anticoagulant
Standard of protein
10 g/dl
Normal range of protein
6.6-8.3 g/dl
Precaution of protein
In vitro
Protein reagent contains
Potassium sodium tartrate and potassium iodide
Total protein formula
Au/As x 10
Sample Stability of protein 1 week
Room temp
Sample Stability of protein 30 days
2-8C
Principle reagent test
Copper rgt. —> sample—> color change —> measurement
Mixture of protein copper reagent
Copper and alkaline solution
