Yotal Protein

Question 1

Method is based on the reports of
__________ and __________ et al.

Answer 1

weischelbaum and gornal et al.

Question 2

the simplest of such compounds formed by heating urea term as

Answer 2

Biuret reaction

Question 3

The reagent used is

Answer 3

Copper sulfate pentahydrate

Question 4

Color produce total protein

Answer 4

Violet

Question 5

Wavelength: Measured at

Answer 5

550nm

Question 6

Rgt storage & Stability: kit

Answer 6

2-8C

Question 7

Rgt storage & Stability: reagent

Answer 7

2-30C

Question 8

Rgt storage & Stability: standard

Answer 8

2-8C

Question 9

Spx collection or sample used

Answer 9

Serum only

Question 10

Why does protein don’t used plasma as sample

Answer 10

It can result increase

Question 11

Interfering Substances of total protein

Answer 11

Gross hemolysis & lipemia.

Question 12

Interfering substances that don’t used in total protein

Answer 12

Urine and spinal fluid (decreased protein

Question 13

Stand cuvets for atleast

Answer 13

5 mins

Question 14

Read total protein within

Answer 14

1 hour

Question 15

Anticoagulant used of total protein

Answer 15

No anticoagulant

Question 16

Standard of protein

Answer 16

10 g/dl

Question 17

Normal range of protein

Answer 17

6.6-8.3 g/dl

Question 18

Precaution of protein

Answer 18

In vitro

Question 19

Protein reagent contains

Answer 19

Potassium sodium tartrate and potassium iodide

Question 20

Total protein formula

Answer 20

Au/As x 10

Question 21

Sample Stability of protein 1 week

Answer 21

Room temp

Question 22

Sample Stability of protein 30 days

Answer 22

2-8C

Question 23

Principle reagent test

Answer 23

Copper rgt. —> sample—> color change —> measurement

Question 24

Mixture of protein copper reagent

Answer 24

Copper and alkaline solution

Question 25

Standard and sample (ul/ml)

Answer 25

0.01ml (10ul)