Albumin

Question 1

Serum albumin The method is based on the modified technique developed by ______, _______, & _______.

Answer 1

Dumas, Watson, & Biggs.

Question 2

It uses special due that binds to serum albumin causing color change

Answer 2

BROMCRESOL GREEN

Question 3

Buffer used in albumin

Answer 3

Citrate buffer

Question 4

Other buffer used in albumin

Answer 4

Succinate buffer

Question 5

Buffer concentration albumin

Answer 5

Lower

Question 6

Albumin Wavelength: Measured at

Answer 6

550nm

Question 7

Albumin Rgt storage & Stability: reagent

Answer 7

2-30C

Question 8

Rgt storage & Stability: standard

Answer 8

2-8C

Question 9

Spx collection or sample used

Answer 9

Serum and plasma

Question 10

Anticoagulants used in albumin

Answer 10

Ethylenediaminetetraacetic acid/ heparin

Question 11

Albumin read within

Answer 11

15mins

Question 12

Linearity of albumin

Answer 12

7.0 g/dl

Question 13

Standard of albumin

Answer 13

6.0 g/dl

Question 14

Normal range of albumin

Answer 14

3.8-5.1g/dl

Question 15

Bcg is sensitive than

Answer 15

Methylene orange and HABA

Question 16

Albumin rgt contains

Answer 16

Surfactant

Question 17

Albumin std added preservatives

Answer 17

Sodium azide

Question 18

Precaution of albumin

Answer 18

In vitro

Question 19

Globulin formula

Answer 19

Total protein - albumin

Question 20

Normal range of globulin

Answer 20

2.0-3.0 g/dl

Question 21

Buffer pH

Answer 21

42 + 0.05

Question 22

BCG REPORTED IN YEAR

Answer 22

1964

Question 23

Sample Stability of albumin 1 week

Answer 23

Room temp

Question 24

Sample Stability of albumin 1month

Answer 24

2-8C

Question 25

This dye binding technique was again published a year later by

Answer 25

Rodkey

Question 26

reported BCG to be more sensitive for albumin than were either methyl orange or HABA
(2-(4’-hydroxyazobenzene)-benzoic acid), two compounds also used in serum albumin quantitation by dye binding.

Answer 26

Watson

Question 27

In 1964 bromocresol green (BCG) was reported as useful in the quantitative determination of serum albumin by

Answer 27

Bartholomew
Delaney
Rodkey

Question 28

BCG method lincarity up to S
g/dL, minimum interference by lipids, hemoglo-
bin and bilirubin, further evidence of high specificity and sensitivity, as well as excellent correlation for albumin values between BCG techniques and electrophoresis. Reported by

Answer 28

Dow
Pinto
Miyada

Question 29

Formula used for albumin

Answer 29

Au/ As x 6.0

Question 30

Normal value for albumin

Answer 30

3.8-5.1 g/dl

Question 31

Color result of albumin

Answer 31

Green

Question 32

Interfering substances of albumin

Answer 32

Lipid (decreased)
Bilirubin (decreased)
Hemoglobin (decreased)

Question 33

Standard and sample (ul/ml) for albumin

Answer 33

0.01ml (10ul)