Non Protein Nitrogen

Question 1

originated in the early days of clinical
chemistry when analytic methodology required removal of protein from a
specimen before analysis.

Answer 1

Nonprotein nitrogen

Question 2

Major excretory product of protein
metabolism

Answer 2

Urea

Question 3

waste product product protein

Answer 3

Urea

Question 4

principal of waste product muscle

Answer 4

Creatinine

Question 5

enzymatic phosphate

Answer 5

Creatine

Question 6

Importance detects renal function

Answer 6

Urea

Question 7

It is also the first metabolite
to increase in kidney disease,

Answer 7

Blood urea nitrogen ( BUN)

Question 8

It is used as a screening test for kidney
disease.

Answer 8

BLOOD UREA NITROGEN ( BUN)

Question 9

is readily removed by dialysis.

Answer 9

Urea

Question 10

Major organic solid in the urine

Answer 10

Blood urea nitrogen ( BUN)

Question 11

CLINICAL APPLICATION BUN

Answer 11

 evaluate renal function,
 to assess hydration status,
 to determine nitrogen balance,
 to aid in the diagnosis of renal disease, and
 to verify adequacy of dialysis

Question 12

Mixture of sulfuric acid and phosphoric acid

Answer 12

Kjeldahl process

Question 13

Oldest method BUN

Answer 13

MICRO-KJELDAHL NESSLER

Question 14

Major inorganic solid in the urine

Answer 14

Chloride

Question 15

Urea measured wavelength at

Answer 15

340 nm

Question 16

Urease Berthelot Method end color

Answer 16

indophenol blue

Question 17

Urease nessler method chemical used

Answer 17

Urease

Question 18

Enzymatic method complex enzymes

Answer 18

L glutamate dehydrogenase

Question 19

Enzymatic method convert

Answer 19

NADH AND NAD

Question 20

Urea convert carbon dioxide and ammonia

Answer 20

Urease berthelot method

Question 21

Yellow diazine derivatives (yellow) called

Answer 21

Fearson reaction

Question 22

Urea is made to react with diacetyl
monoxime to produce a yellow diazine
derivative (Fearon’s reaction).

Answer 22

Diacetyl Monoxime Method (DAM)

Question 23

containing anticoagulants are
contraindicated in enzymatic methods.

Answer 23

Ammonium oxalate

Question 24

inhibits the action of
urease.

Answer 24

Sodium fluoride and citrate

Question 25

Upon prolonged standing, ammonia concentration in the sample rises 2-3 times the original value due to enzymatic deamination of labile amide like

Answer 25

glutamine

Question 26

NORMAL VALUE: BUN

Answer 26

6-20 mg/dl

Question 27

24hr urine: BUN

Answer 27

12-20 mg/dl

Question 28

Conversion factor of bun

Answer 28

0.357

Question 29

Urea nitrogen concentration can be converted to urea concentration by multiplying by

Answer 29

2.14

Question 30

Urea nitrogen concentration expressed in milligrams per deciliter may be converted to urea concentration in millimoles per liter by multiplying by

Answer 30

0.167

Question 31

It is synthesized form three amino acids of creatinine

Answer 31

methionine, arginine and lysine

Question 32

Fetal kidney maturity

Answer 32

Creatinine

Question 33

Urease came from

Answer 33

Jack bean

Question 34

- production of constant

Answer 34

Creatinine

Question 35

Not easily removed by Dialysis

Answer 35

Creatinine

Question 36

The most commonly used assay for Renal Blood Flow

Answer 36

Creatinine

Question 37

GFR HIGH CREATININE IS

Answer 37

Low

Question 38

Available on Ektachem analyzer

Answer 38

Creatinase method

Question 39

Oxidation of indicator in creatinase

Answer 39

Hydrogen peroxide

Question 40

Creatinine is hydrolyzed to creatine by creatinine aminohydrolase followed by a series of coupled enzyme reactions in which creatine reacts with creatinine kinase, pyruvate kinase, and lactate dehydrogenase, culminating in the oxidation of the NADH.

Answer 40

Creatinine Aminohydrolase Method

Question 41

PRINCIPLE OF CREATININE DETERMINATION:

Answer 41

Jaffe reaction

Question 42

Jaffe reagent

Answer 42

Picric acid + sodium hydroxide = alkaline solution

Question 43

Jaffe end color

Answer 43

Red orange

Question 44

Absorbent of creatinine

Answer 44

Lloyds reagent Fuller earth reagent

Question 45

Lloyd’s Reagent is

Answer 45

sodium aluminum silicate

Question 46

Fuller Earth’s Reagent is

Answer 46

aluminum magnesium silicate

Question 47

Conversion factor of creatinine

Answer 47

88.4

Question 48

Colorimetric of Simple and Non specific

Answer 48

Endpoint

Question 49

Colorimetric of Rapid and increased specificity

Answer 49

Kinetic

Question 50

CLINICAL SIGNIFICANCE: creatinine

Answer 50

 Muscular dystrophies  Familial periodic paralysis  Myasthenia gravis  Dermatomyositis

Question 51

defined as the increased in urea and creatinine (azotemia) with accompanying clinical signs and symptoms of renal failure

Answer 51

Uremia

Question 52

defined as the increased in urea and creatinine (azotemia) with accompanying clinical signs and symptoms of renal failure

Answer 52

Uremia