Worksheet 3-1

Question 1

two common in house immunologic assays used in vet practice

Answer 1

ELISA
CELISA

Question 2

tests for monoclonal antibodies or specific antigen

Answer 2

ELISA

Question 3

tests for monoclonal antibodies and enzyme labeled antigen

Answer 3

CELISA

Question 4

card test to blood type dogs

Answer 4

DEA 1 card test

Question 5

what dog blood type will react with antiserum and will cause agglutination

Answer 5

DEA 1 - positive

Question 6

what dog blood type will not have a reaction

Answer 6

DEA 1 - negative

Question 7

What does the reference lab Coombs test used to confirm in small animals?

Answer 7

Immune mediated hemolytic anemia (IMHA)

Question 8

What is the most common DNA reference lab test?

Answer 8

PCR (polymerase chain reaction) test

Question 9

What are the two most-used tests which use intradermal injections in veterinary medicine.

Answer 9

Allergy testing and TB testing

Question 10

What are the three basic elements evaluated in a complete urinalysis?

Answer 10

Physical
Chemical
Formed (Sediment)

Question 11

What are the three physical characteristics of urine that are evaluated in a complete urinalysis?

Answer 11

Color
Clarity
Specific Gravity

Question 12

how many types of hypersensitivies are there

Answer 12

4

Question 13

hypersensitivity type:

immediate, occurs when mast cells release chemical mediators.

Answer 13

Type 1

Question 14

what hypersensitivity type is anaphylactic shock

Answer 14

Type 1

Question 15

hypersensitivity type:

antibodies are directed at the animals own cells

Answer 15

Type 2

Question 16

what hypersensitivity type is IMHA

Answer 16

Type 2

Question 17

hypersensitivity type:

Immune Complex Disease, antibodies and antigens form complexes that are deposited in blood vessels

Answer 17

Type 3

Question 18

what hypersensitivity type is Lupus

Answer 18

Type 3

Question 19

hypersensitivity type:

reaction of T cells against self antigens

Answer 19

Type 4

Question 20

what hypersensitivity type is plastic food bowls

Answer 20

Type 4

Question 21

The ability of the test to correctly id all animals that are truly positive (minimal false negatives)

Answer 21

Sensitivity

Question 22

The measure of false positives, does the test evaluate a given parameter correctly (minimal false positives)

Answer 22

Specificity

Question 23

cloudy or opaque, used when describing urine clarity

Answer 23

turbidity

Question 24

looks like tufts of wool, clumped

Answer 24

flocculent

Question 25

True / False. You don’t have to use a refractometer to measure USG if you are doing a full urinalysis because USG is one of the things the reagent strips test for.

Answer 25

False – reagent strips are the least reliable method so you want to also use the refractometer.

Question 26

A urine sample should be analyzed within what time after collection?

If you can’t perform the urinalysis in this time frame, what step can you take to help it stay fresh?

Answer 26

Within 30 minutes to an hour, put in fridge for up to 6 to 12 hours.

Question 27

What is the preferred method of urine sample collection for urinalysis, especially if you want to do a culture and sensitivity?

Answer 27

cystocentesis