WOLBACHIA METHOD

Question 1

The main focus of WMP is to replace native mosquito populations with Wolbachia-infected ones.

Answer 1

TRUE

Question 2

The WMP uses ground-based stationary release methods exclusively for distributing Wolbachia-infected mosquitoes.

Answer 2

FALSE

Question 3

Aedes aegypti mosquitoes are the primary vector for dengue virus transmission.

Answer 3

TRUE

Question 4

The entire viral replication and maturation cycle in a mosquito takes approximately 2–3 days.

Answer 4

FALSE

Question 5

Wolbachia is an intracellular bacterium that infects arthropods, including mosquitoes.

Answer 5

TRUE

Question 6

Wolbachia disrupts the ER’s lipid synthesis and trafficking, impairing the formation of replication complexes essential for DENV.

Answer 6

TRUE

Question 7

Wolbachia is transmitted vertically from female mosquitoes to their offspring.

Answer 7

TRUE

Question 8

Wolbachia infections cause an impact on mosquito fitness.

Answer 8

FALSE

Question 9

Cytoplasmic incompatibility prevents the survival of offspring from infected males and uninfected females.

Answer 9

TRUE

Question 10

Cytoplasmic incompatibility caused by Wolbachia gives a reproductive advantage to infected females.

Answer 10

TRUE

Question 11

Wolbachia promotes the production of antimicrobial peptides like defensins and cecropins in mosquitoes.

Answer 11

TRUE

Question 12

The IMD pathway is activated by REL2-ANK phosphorylation, which blocks the transcription of immunerelated genes.

Answer 12

FALSE

Question 13

Overactivation of immune pathways caused by Wolbachia is balanced by miRNAs like aae-miR-34-3p.

Answer 13

TRUE

Question 14

The wMel strain of Wolbachia can be cultured outside cells, as it is not dependent on an intracellular environment.

Answer 14

FALSE

Question 15

Cytoplasmic incompatibility depends on proteins CifA and CifB encoded by Wolbachia.

Answer 15

TRUE

Question 16

AMPs like cecropins and defensins harm both dengue virus and Wolbachia within the mosquito.

Answer 16

FALSE

Question 17

Wolbachia disrupts mosquito autophagy, reducing the availability of free fatty acids needed by the dengue virus.

Answer 17

TRUE

Question 18

MicroRNA aae-miR-2940, regulated by Wolbachia, promotes its maintenance in mosquito populations.

Answer 18

TRUE

Question 19

Wolbachia infection in Aedes aegypti negatively impacts autophagy by regulating aae-miR-12.

Answer 19

TRUE

Question 20

During embryonic microinjection, bacteria are injected into the anterior end of mosquito embryos.

Answer 20

FALSE