DERMOKINE

Question 1

ORF genetics specializes in producing recombinant proteins using genetically modified rice.

Answer 1

FALSE

Question 2

ORF Genetics uses geothermal energy to power its greenhouses.

Answer 2

TRUE

Question 3

ORF Genetics’ proprietary OrfeusTM system is specifically designed for bacterial protein expression.

Answer 3

FALSE

Question 4

The transformation vector used in ORFEUS contains a promoter that drives expression specifically in barley leaves.

Answer 4

FALSE

Question 5

Barley cultivation for recombinant protein production can be performed only in very specific regions of the world.

Answer 5

FALSE

Question 6

BIOEFFECT products are derived from barley and aim to restore EGF levels in the skin.

Answer 6

TRUE

Question 7

The JAK-STAT pathway regulates the proliferation of keratinocytes.

Answer 7

FALSE

Question 8

Barley is genetically modified by inserting a gene into its chloroplasts.

Answer 8

FALSE

Question 9

Barley embryos are transformed and grown in a selective medium to identify successful
transgenic lines.

Answer 9

TRUE

Question 10

A. rhizogenes is used in the ORFEUS technology for genetic transformation.

Answer 10

FALSE

Question 11

Agrobacterium uses a binary plasmid system in biotechnology applications to insert the gene
of interest.

Answer 11

TRUE

Question 12

Agrobacterium tumefaciens inserts T-DNA into the host plant genome using a type VI
secretion system.

Answer 12

FALSE

Question 13

Vir genes on the Ti plasmid are responsible for plant virulence.

Answer 13

TRUE

Question 14

The T-DNA region in Agrobacterium’s Ti plasmid contains genes that cause tumor formation
in plants.

Answer 14

TRUE

Question 15

The binary plasmid system involves one plasmid containing oncogenes and another
containing T-DNA with the gene of interest.

Answer 15

FALSE

Question 16

Electroporation is a method used to introduce binary plasmids into Agrobact cells.

Answer 16

TRUE

Question 17

Proteins produced using ORFEUS technology are purified directly from barley roots.

Answer 17

FALSE

Question 18

Endotoxin contamination more likely in recombinant prot prod in E. coli than in barley.

Answer 18

TRUE

Question 19

Plant-based systems, like ORF’s barley method, reduce the carbon footprint compared to
bioreactor systems.

Answer 19

TRUE