REVIVICOR UHeart

Question 1

Organ transplantation is the most promising option for treating end-stage organ failure.

Answer 1

TRUE

Question 2

Revivicor focuses on producing genetically modified cows for xenotransplantation.

Answer 2

FALSE

Question 3

In 2022, a UHeartTM transplant set a record for the longest surviving xenotransplantation recipient.

Answer 3

TRUE

Question 4

Revivicor’s GalSafeTM pigs are approved only for human food consumption, not for therapeutics.

Answer 4

FALSE

Question 5

The “10 GE pigs” contain 6 deleted genes and 4 human genes inserted into their genome.

Answer 5

FALSE

Question 6

The inactivation of GGTA1 in pigs completely ensures the compatibility of their organs in humans, by not expressing the α-Gal antigen.

Answer 6

FALSE

Question 7

Differences between the porcine SDa antigen and the similar human antigen prevent the production of anti-SDa antibodies.

Answer 7

FALSE

Question 8

Humans naturally express Neu5Ac, which prevents any immune response against this antigen.

Answer 8

TRUE

Question 9

Organs from GHR-KO pigs are more compatible in size with humans and may have lower metabolic demands.

Answer 9

TRUE

Question 10

They saw in NHP studies that CD46 and DAF greatly prolong xenograft survival.

Answer 10

TRUE

Question 11

When Protein C binds to EPCR, Protein C is inactivated leading to the a procoagulant effect.

Answer 11

FALSE

Question 12

The increase of expression of HO1 extends xenograft survival.

Answer 12

TRUE

Question 13

CD47 is selectively expressed in human cells.

Answer 13

FALSE

Question 14

The genetically modified pigs contain 4 insertions and 6 deletions.

Answer 14

FALSE

Question 15

The technology used to generate the transgenic pigs are CRISPR-Cas9 and Multicistronic vectors.

Answer 15

TRUE

Question 16

Revivicor uses 3 bicistronic vectors, and each of them contains 2 of the inserted genes.

Answer 16

FALSE

Question 17

The element that allows them to select the genetic modified cells is the Neomycin resistance
gene.

Answer 17

TRUE

Question 18

eGenesis uses an alternative approach that consists on the inactivation of the 59 PERVs of
the porcine xenograft by CRISPR techniques.

Answer 18

TRUE

Question 19

One of the primary ethical concerns regarding xenotransplantation is the potential for
zoonotic diseases to spread to human.

Answer 19

TRUE

Question 20

Fibrosis is a key feature of chronic rejection due to the buildup of scar tissue that causes the transplanted organ to become stiff.

Answer 20

TRUE