Wk8 - communication Flashcards
Cells are believed to communicate in 3 ways; state them.
- Gap junctions.
- Plasma-membrane bound signalling molecules (influence cells in direct contact).
- Secretion of chemicals (influence cells some distance away).
Chemical communication occurs in 3 ways; state and briefly explain them.
- Paracrine signalling - local chemical mediators, only act on cells in immediate vicinity.
- Endocrine signalling - specialised endocrine cells release hormones which travel through the blood, influencing target cells far away.
- Neurotransmitters - nerve cells release NT into chemical synapses, which are received by the target cell.
Contrast between paracrine and autocrine signals.
Paracrine chemical signals are those chemical mediators which act on cells in the immediate vicinity.
Autocrines are a subdivision of paracrine signals, which involve chemical mediators acting only on the SAME TYPE of cell which released them.
What structure(s) are known for to quickly destroy paracrines?
Extracellular enzymes.
t/f: a chemical may, in some circumstances, act as both a paracrine and an autocrine signal.
true.
What forms paracrines/ autocrines?
- Proteins (ie. cytokines).
2. Steroids.
Explain what is meant by the following term(s):
Cytokines.
Cytokines are chemical messengers (paracrines) produced by proteins, which play an important role in the immune response.
Explain what is meant by the following term(s):
Eicosanoids.
Eicosanoids are paracrines derived from cell membrane lipids, they come in 3 classes.
What’re the 3 classes of eicosanoids?
- Prostaglandins.
- Leukotrienes.
- Thromboxanes.
Some cells are specialised for paracrine signalling. Provide an example of this.
- Mast cells (found in CT) secrete histamine.
- Histamine is stored in large secretory vesicles, which can be rapidly released.
- Histamine causes local BV to dilate and become leaky.
How often are prostaglandins released in mammals? How often are they degraded - and by what?
Prostaglandins are continuously released in mammalian tissues. They are also continuously degraded by enzymes in the ECF.
State the derivative structure of prostaglandins.
Prostaglandins are 20C fatty acid derivatives of ARACHIDONIC acid.
How many different types of prostaglandins (PG) exist; and how many ‘classes’ do these fall into?
There are 16 or more PG’s, which fall into 9 classes. The different types of PG’s produce different biological responses as they bind to different cell surface receptors.
(general ass vague ass info but wtv phil).
What causes increased PG secretion?
Cells are activated to increase PG synthesis and release due to:
- Local tissue damage.
- Chemical stimuli.
State the general known biological activities of PG’s. (4)
- Inflammation.
- Contraction of SM.
- Platelet aggregation.
- Contraction of uterine SM.
What may reduce inflammation which is brought about by increased levels of PG’s?
The drug aspirin inhibits PG synthesis, and so reduces inflammation.
State Nitric Oxide’s (NO)…
a. ) Structure.
b. ) Permeability.
c. ) Potency.
a. ) Structure - small, gaseous.
b. ) Permeability - membrane permeable (lipophillic).
c. ) Potency - high (powerful biological effects).
What is the half life of NO?
2-30 seconds (very short!).
What enzyme is responsible for the local synthesis of NO? What substances are required for this synthesis?
Nitric oxide synthase (enzyme) produces NO. It requires:
- Ca+2 (cofactor)
- calmodulin (cofactor)
- L-arginine (substrate)
In what cell is NO synthesized?
Vascular endothelial cells.
State the basic sequence of events involved with NO synthesis. (4)
- Stimuli such as ACh, bradykinin, thrombin, adenine nucleotides or Ca+2 bind to ligand receptor on endothelial cell.
- These substances trigger entry of Ca+2 into the cell.
- Ca+2 binds to cytosolic calmodulin, which then activates enzyme NOS.
- NOS produces NO, which rapidly diffuses out of the endothelial cell and crosses the membrane of neighbouring SM cell.
Synthesis of NO from NOS within a vascular endothelial cell is triggered by certain substances binding to ligand specific receptors on the membrane. State such stimulants. (5)
- ACh
- Ca+2
- Bradykinin
- Thrombin
- Adenine nucleotides
List the 6 physiological effects of NO.
“LICCIN”
L - locally produced NT.
I - Immunity; released by WBC to kill bacteria/ tumour cells.
C - control BF/ pressure.
C - control renin release (JG cells).
I - Inhibit platelet aggregation.
N - Neural NT; may play role in learning/ memory.
Explain a clinical use of NO.
NO used to treat angina pectoris (chest pain due to coronary heart disease). NO relaxes SM of peripheral arterioles, reducing work of the heart and relieving associated pain.
As a NT, is NO released from presynaptic or postsynaptic nerves?
Both.
t/f: NO is contained within secretory vesicles within the pre-synaptic nerve terminals.
FALSE - NO is not contained within vesicles.
NO is not contained within secretory vesicles; what is the functional consequence of this?
As NO is not contained within secretory vesicles it does not require Ca+2 to stimulate its release.
Why is NO not considered to be a “normal” NT?
- NO isn’t stored within vesicles and thus doesn’t require Ca+2 to stimulate it’s release.
- NO doesn’t bind to any receptor on the surface of its target cell; it diffuses freely into the cell.
Endocrine cells rely on the diffusion of their chemical messengers in the blood. What is the functional consequence of this?
As substances must travel within the bloodstream, they are therefor heavily diluted. This means they must be able act at very low concentrations (<10^-8 M).
Endocrine chemicals must be able to act at very low concentrations. At the target tissue end of things, how are endocrine hormones received?
AMPLIFICATION - a single hormone molecule interacts with a receptor, leading to the formation of many active protein products.
The ability of a cell to respond to extracellular chemicals depends on the possession of what structural feature?
Substance-specific ligand receptors. These bind to their signalling molecules with high affinity.