WK - Microbial Diversity Flashcards

1
Q

What are 2 characterisitcs of Early Earth?

A
  • Occurred about 4.5 billion years ago
  • Highly anaerobic and reducing world
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2
Q

What are some features of Early Microbes? (5)

A
  • Anaerobic microbes that could tolerate extreme environments (e.g. deep-sea vents)
  • A number of single-cell bacterium-like organisms
  • Likely living off of H2 and S
  • Probably hyperthermophilic
  • Can withstand the very high temperatures in boiling muds
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3
Q

What is an example of one of these early microbes?

A

LUCA

  • The organism from which the evolution of life as we know it began
  • Two-domain system (eukaryotes within archaea)
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4
Q

What is the significance of endosymbiosis in microbial evolution? (3)

A
  • Mitochondria evolved from Alphaproteobacteria (rickettsia bacteria)
  • Nucleus may have originated from archaea
  • Chloroplast evolved from cyanobacteria (photosynthesis)
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5
Q

What are 3 microbial prokaryotic groups?

A
  • Myxobacteria
  • Spirochetes
  • Haloquadratum
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6
Q

Who discovered a new domain of life using 16S rRNA genes?

A

Carl Woese (1977)

Used molecular biology to identify archaea as a new domain of life

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7
Q

What are 2 microbial eukaryotic groups?

A
  • Fungi
  • Protists
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8
Q

What distinguishes archaea from bacteria? (3)

A
  • Thrive in extreme environments (hot springs, salt lakes)
  • Have ether-linked lipids
  • Only a small number have been studied in the lab
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9
Q

Bacterial Groups (6)

A

1) Actinobacteria

2) Wolbachia

3) Nitrogen Cyclers:

  • Nitrogen fixers
  • Ammonia-oxidisers
  • Nitrite-oxidisers
  • Denitrifiers

4) Spirochetes
5) Cyanobacteria
6) Bdellovibrionota

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10
Q

What conditions can archaea survive in? (3)

A
  • Extreme high temperatures
  • High salinities
  • Anaerobic environments
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11
Q

What are some features of methanogens? (4)

A
  • Strict anaerobes
  • Found in anoxic environments (e.g. sediments, animal guts)
  • Perform methanogenesis, producing methane as a byproduct
  • Belong to the euryarchaeota
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12
Q

What are asgard archaea?

A

Believed to be the branch that led to eukaryotes

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13
Q

What are the two main morphological types of fungi?

A

1) Yeasts (unicellular) – usually asexual budding

2) Filamentous (hyphal / mycelium) – sexual growth

  • Mushrooms (macroscopic fruiting bodies)
  • Tubular, elongated, and filamentous structures, which can contain multiple nuclei
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14
Q

What are some major fungal groups? (4)

A

Ascomycota

  • Sac fungi
  • Most diverse and most heavily studied from a diversity context

Basidiomycota

  • Club fungi
  • Many are ectomycorrhizal fungi

Glomeromycota

  • Very ancient fungal lineage
  • Obligate symbionts with plants (~90% of plants)
  • Responsible for terrestrialisation

Chytridiomycota

  • Earliest diverging fungal lineages
  • Motile spores (need moisture)
  • Chytridiomycosis - Devasting amphibian populations worldwide
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15
Q

What are the 4 types of protist groupings?

A

Flagellates

  • Flexible flagella that beat back and forth
  • Free-living predators
  • Giardia

Amoebae

  • Amorphous (lack shape)
  • Free-living predators
  • Pseudopodia (false feet) for motility

Ciliates

  • Cilia for locomotion
  • Microbial predators

Stationary (Sporozoa)

  • Some strictly parasitic nonmotile protozoans
  • Non-motile spores but some have undulating ridges
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16
Q

What is the role of protist algae in ecosystems? (2)

A
  • Photosynthetic, containing chlorophyll a
  • Base of aquatic food-webs, with a significant impact on the global carbon cycle
17
Q

Who first observed bacteria and protists using a microscope?

A

Antonie van Leeuwenhoek

  • Observed them in the 1670s and called them “animalcules”
18
Q

What method did Louis Pasteur and Robert Koch pioneer for studying microbes? (2)

A

Louis Pasteur – Liquid culture (1860s)
Robert Koch – Solid media (1860s)

19
Q

What method did Sanger and Illumina use to study microbes?

A

High-throughput technologies