WK 8: Lab evaluation of 2nd Hemostasis

Question 1

Specimen of choice for secondary hemostasis

Answer 1

Anticoagulated whole blood

Question 2

Anticoagulant used:

Answer 2

Sodium citrate (3.2%)

Question 3

(True or False)

Blood:Citrate ration is 1:9

Answer 3

(False)

9:1

Question 4

This drug suppresses the platelet function

Answer 4

Aspirin

Question 5

This drug reduces activities of Factor II, VII, IX, X

Answer 5

Coumadin (Warfarin)

Question 6

(True or False)

Remove tourniquet within 1 minute of application

Answer 6

True

Question 7

(True or False)

PTT is stored at 18-24C and processed within 24 hours

Answer 7

(False)
PPT = 4 hours
PT = 24 hours

Question 8

Plasma stored at -20C should be processed within how many weeks?

Answer 8

2-4 weeks

Question 9

Frozen samples thawed at 37C should be tested within how many hours?

Answer 9

1 hour

Question 10

If the centrifugation is <10 minutes, what component may be produced?

Answer 10

Platelet rich plasma

Question 11

(True or False)

When the hematocrit level is <55%, anticoagulant reduction must be done

Answer 11

(False)

>55%

Question 12

This test is for Extrinsic pathway deficiencies

Answer 12

PT

Question 13

Reagent used in Prothrombin time

Answer 13

Thromboplastin

Question 14

The buffer used in PT

Answer 14

Calcium chloride

Question 15

It indicates the purity of the reagent

Answer 15

ISI (Internationat sensitivity index)

Question 16

Test that induces clot plasma formation without added thromboplastin

Answer 16

APTT

Question 17

(True or False)

PT is for Intrinsic factor deficiencies

Answer 17

(False)
PT = Extrinsic
APTT = Intrinsic

Question 18

This test is for monitoring heparin therapy

Answer 18

APTT

Question 19

This test is for monitoring warfarin therapy

Answer 19

PT

Question 20

Reagent used in APTT

Answer 20

Partial thromboplastin

Question 21

3 clotting factors that can activate clot formation in vitro thru intrinsic pathway

Answer 21

Factor XIIa
HMWK
Pre-K

Question 22

Normal range for APTT

Answer 22

25-40 seconds

Question 23

Therapeutic range for APTT

Answer 23

60-100 seconds

Question 24

Produces clot plasma formation after the addition of thrombin in the presence of calcium

Answer 24

Thrombin clotting time (TCT)

Question 25

2 heparin-like substances

Answer 25

Fibrin degradation products | Plasma proteins

Question 26

It is a quantitative/qualitative test for fibrinogen

Answer 26

Thrombin clotting time (TCT)

Question 27

Reagent used in TCT

Answer 27

Thrombin

Question 28

Thrombin cleaves _____ & ______

Answer 28

fibrinopeptides A & B

Question 29

Normal range for PT

Answer 29

10-14 seconds

Question 30

Normal range for TCT

Answer 30

15-20

Question 31

(True or False) | Dysfibrinogenemia is when you have sufficient fibrinogen but is dysfunctional

Answer 31

True

Question 32

(True or False) | Hypofibrinogenemia means you have high values of fibrinogen

Answer 32

(False) | Low

Question 33

This test converts fibrinogen to fibrin with the use of thrombin-like enzyme

Answer 33

Reptilase time

Question 34

This test is a modification of TCT but uses a different reagent

Answer 34

Reptilase time

Question 35

Thrombin-like enzyme used in Reptilase time came from the venom of what reptile?

Answer 35

Bothrops atrox

Question 36

It is also known as Stypven time

Answer 36

Russel Viper Venom test (RVVT)

Question 37

This test is an alternative for PT

Answer 37

RVVT

Question 38

Reagent used in RVVT came from the venom of what reptile?

Answer 38

Daboia russelii

Question 39

This test is used to detect the reason for the prolonged clotting time

Answer 39

Mixing studies

Question 40

(True or False) | When the prolonged clotting time is corrected, this is due to Factor inhibitor

Answer 40

(False) Corrected = Factor deficiency Not corrected = Factor inhibitor

Question 41

This test is a quantitative test for fibrinogen

Answer 41

Fibrinogen assay

Question 42

Buffer used in Fibrinogen assay

Answer 42

Owren buffer

Question 43

Prolonged PT, PTT | Normal TCT

Answer 43

Factor VII deficiency

Question 44

Normal PT PTT, Fibrinogen level

Answer 44

Factor XIII deficiency

Question 45

Assay used to measure the release of ammonia during an in vitro transglutaminase reaction

Answer 45

Factor XIII assay

Question 46

It is a fibrin degradation product

Answer 46

D-dimer

Question 47

In D-dimer immunoassay, the latex particles are coated with ________ antibodies with patient's plasma D-dimer

Answer 47

Monoclonal anti-D dimer

Question 48

The endpoint reaction of this test yields a color whose intensity id directly proportional to the activity of serine protease

Answer 48

Chromogenic assay

Question 49

This test is a Factor XIII screening test

Answer 49

Urea Solubility test