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HEMA 1: LAB > White Blood Cell Microscopic Identification > Flashcards

White Blood Cell Microscopic Identification Flashcards

1
Q

• A heterogenous group of nucleated cells found in the blood circulation

A

White Blood Cells (leukocytes)

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2
Q

WBCs
• Play an important role in_______ of the body

A

immunity

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3
Q

• Classifications

A

Granulocyte
Agranulocyte

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4
Q

Granulocyte

A

Neutrophil
Eosinophil
Basophil

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5
Q

Agranulocytes

A

Lymphocyte
Monocyte

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6
Q

NEUTROPHIL
• In the circulation:
• Size:

A

Mainly mature segmented neutrophils

10-14 um

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7
Q

NEUTROPHIL

• Nucleus:
• Cytoplasm:

A

Central or eccentric blue violet 3-4 lobes, connected by chromatin thread

Pale blue to pink

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8
Q

NEUTROPHIL

• Nucleus: Central or eccentric (color and number of lobes), connected by_____

• Cytoplasm: Pale blue to pink

A

blue violet 3-4 lobes

chromatin thread

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9
Q

NEUTROPHIL

Cytoplasmic granules

A

Azurophilic, fine, closely packed, violet-pink/ fine lilac to pink, not seen separately, and do not cover the nucleus

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10
Q

Azurophilic, fine, closely packed, violet-pink/ fine lilac to pink, not seen separately, and do not cover the nucleus

A

Neutrophil

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11
Q

NEUTROPHIL

• Function: Defense against ______
• Life span: ______ (circulation); _______(tissues)

A

Defense against bacterial
infection (phagocytosis)

6-10 hrs (circulation);
5 days (tissues)

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12
Q

• Function: Defense against bacterial
infection (phagocytosis)
• Life span: 6-10 hrs (circulation); 5 days (tissues)

A

Neutrophil

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13
Q

immature neutrophil

A

Band cell

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14
Q

BAND CELLS

• In the circulation:
• Size:

A

small in number

10-14 um

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15
Q

BAND CELLS

• Nucleus:
• Normally account for approx.____ of peripheral blood leukocytes

A

non-segmented, C or S shaped nuclei

5-10%

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16
Q

• Nucleus; non-segmented, C or S shaped nuclei
• Normally account for approx. 5-10% of peripheral blood leukocytes

A

Band cells

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17
Q

NEUTROPHIL GRANULES

A

• Proteases
• Oxidizing agents
• NADPH oxidase
• Defensins
• Lysozymes
• Myeloperoxidases

18
Q

• BI-LOBED

A

EOSINOPHIL

19
Q

EOSINOPHIL

• Size:
• Nucleus:
• Cytoplasm:

A

10-14 um

Central or eccentric purplish blue 2 lobes, connected by a thick chromatin thread, “spectacle shaped”

acidophilic, light pink to red

20
Q

• Size: 10-14 um

• Nucleus: Central or eccentric purplish blue 2 lobes, connected by a thick chromatin thread,
“spectacle shaped”

• Cytoplasm: acidophilic, light pink to red

A

Eosinophil

21
Q

• Cytoplasmic Granules: Eosinophilic, large, coarse, uniform sized, brick red to orange, seen separately, does not cover nucleus

A

Eosinophil

22
Q

• Function: involved in allergy (neutralize basophil and mast cell products) and parasitic infections

• Life span: 6-10 hrs (circulation); 5 days (tissues)

A

Eosinophil

23
Q

Eosinophil

• Function: involved in ______ (neutralize basophil and mast cell products) and______

• Life span:
_______ (circulation);______(tissues)

A

allergy; parasitic infections

6-10 hrs

5 days

24
Q

EOSINOPHIL GRANULES

A

• Major basic protein
• Acid phosphatase
• Arylsulfatase
• Eosinophilic cationic protein
• Eosinophil derived neurotoxin
• Eosinophil peroxidase

25
Q

BASOPHIL GRANULES

A

• Bradykinin
• Eosinophil chemotactic factor
• Serotonin
• Heparin
• Histamine

26
Q

BASOPHIL
• Size:
• Nucleus:
• Cytoplasm:

A

10-15 um

Central, 2-3 lobes, blue-violet S-shaped, not clearly seen due to overlaying of granules

Bluish, basophilic, full of granules

27
Q

• Size: 10-15 um
• Nucleus: Central, 2-3 lobes, blue-violet S- shaped, not clearly seen due to overlaying of granules
• Cytoplasm: Bluish, basophilic, full of granules

28
Q

• Cytoplasmic Granules: Large, very coarse, variable in size, deep bluish purple, observed separately, completely fill the center and obscure the nucleus

29
Q

Basophil

• Function:

• Life span:

A

Allergic inflammation progression and maintenance

Exist for only a few hours in the bloodstream

30
Q

• Function: Allergic inflammation progression and maintenance

• Life span: Exist for only a few hours in the bloodstream

31
Q

• The smallest WBC
• Same size as the RBCs

A

LYMPHOCYTE

32
Q

Lymphocytes

• Size:_____ (Small lymphocyte);____(Large lymphocyte)

• Nucleus:

• Cytoplasm:

A

7-9 um; 10-15 um

Eccentric, indented, large round nucleus, deep purplish blue

Scanty, sparse, light blue color

33
Q

• Size: 7-9 um (Small); 10-15 um (Large)

• Nucleus: Eccentric, indented, large round nucleus, deep purplish blue

• Cytoplasm: Scanty, sparse, light blue color

A

Lymphocytes

34
Q

• Cytoplasmic Granules: None

• Function: Defense against viral infections, specific acquired immunity, participates in cell-mediated and humoral immunity

A

Lymphocytes

35
Q

3 types of lymphocytes

A

T-cell, B-cell, Natural Killer (NK) cell

36
Q

MONOCYTE

• Circulation:_____
• Tissues:_____

A

Monocyte

Macrophages

37
Q

Monocytes

• Size:

Nucleus:

• Cytoplasm:

A

12-20 um

pale blue violet, large, single, indented or horse shoe or kidney shaped, occupies almost half of the cytoplasm

Dull grayish blue/pale blue;
“ground-glass appearance”; clear, may contain digestive vacuole

38
Q

12-20 um

pale blue violet, large, single, indented or horse shoe or kidney shaped, occupies almost half of the cytoplasm

Dull grayish blue/pale blue;
“ground-glass appearance”; clear, may contain digestive vacuole

39
Q

• Cytoplasmic Granules: Azurophilic, fine lilac granules

40
Q

Monocytes

• Function:

• Life span:

A

Phagocytosis, bacterial killing, antigen presentation

70 hours (peripheral blood)

41
Q

• Function: Phagocytosis, bacterial killing, antigen presentation

• Life span: 70 hours (peripheral blood)

HEMA 1: LAB (13 decks)

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