What would you put up for...? Flashcards
What would you put up for all sputa samples? (4)
Blood agar + optochin
Chocolate + C30
Chocolate dilution plate
1/4 Candida Chromagar
What would you put up for a BAL sample? (6)
Blood + optochin
Chocolate + C30 (NO DILUTION PLATE)
MacConkey
Full Candida chromagar
PDA slope
TB reflex testing
What would you put up for a sputa from a lung transplant patient? (8)
Blood + optochin
Chocolate + C30
Chocolate dilution plate
Own Candida plate
CxSA
CxMRSA
FAA + Metronidazole for anaerobes
Burkholderia plate
What would you put up for a lung transplant BAL? (10)
Blood + optochin
Chocolate + C30 (No dilution)
MacConkey
Own Candida
CxSA
CxMRSA
FAA + Metronidazole
Burkholderia Cepacia
PDA slope (5 days)
Lateral flow for aspergillus
What would you put up for an EBUS sample? (6)
Blood + optochin
Chocolate + C30
Dilution
1/4 Candida
FAA + metronidazole
TB testing (added on)
What would you put up for a Cystic fibrosis sputa sample?
Blood + optochin
Chocolate + C30
Chocolate dilution
1/4 Candida
CxSa
CxMRSA
Burkholderia Cepacia
What would you put up for a sputa sample of a COPD patient?
Blood + optochin
Chocolate + C30
Chocolate dilution
MacConkey
Candida chromagar
PDA slope
What would you put up for a positive blood culture? (5)
Blood + metronidazole (inc anaerobically)
Chocolate (CO2)
MacConkey (aerobic)
Miller Hinton (aerobic)
Gram stain
What direct sens are put up for blood cultures? (6)
○ Gram pos:
▪ Fox (MRSA indicater)
▪ Vanc (VRE indicator)
* Only works on gram positives
* If query staph/yeast look at vanc -> candida will grow right up to vanc
* All GNBs intrinsically resitant to vanc
▪ Cip
○ Gram neg
▪ TZP
▪ Aug
CPD (ESBL indicater)
What would you put up for a CSF?
(3)
Blood
Chocolate
Film array -> no gram
What plates are put up for swabs/wounds?
Blood + C30 (anerobic)
Chocolate
MacConkey
CxSA
NEO
FAA
If query fungal -> PDA plate
Corneal scrapings -> nutrient agar for acantomoeba
No gram for swabs but there is for tissues
What would you put up for fluids?
All = Blood, Choc, MacConkey, CxSA, FAA, NEO
All = Gram stain
Joint fluid might get cooked meat broth
Pleural fluid/joint fluid might get blood cultures
Bile gets a selenite broth
What would you put up a cooked meat broth for?
Heart valves, bone infection, small amount of joint fluid
Any infection where there might be very low bacterial numbers
What would you put up for a ?MRSA?
MRSA Chromagar
Cefoxitin + Oxacillin + Linezolid discs
VITEK Sens
What would you put up for a VRE?
VRE Chromagar
Disc sens = Vancomycin, ampicillin, linezolid, nitro
VITEK Sens
