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Fellowship and boards study > What's my stain, what's my stain > Flashcards

What's my stain, what's my stain Flashcards

1
Q

Alveolar soft part sarcoma

A

TFE3 +, transcription factor PAS-D+ rhomboid crystals

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2
Q

Synovial sarcoma

A

TLE1+; CK+; SSX-SS18 stain

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3
Q

Solitary fibrous tumor

A

STAT6 + BCL2 + CD34 +

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4
Q

Myofibroma

A

SMA+ MSA+ Desmin -

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5
Q

Paraganglioma

A

Chromo +, synapto + main tumor cells Sustentacular cells: s100+ ***same as a pheochromocytoma- these tumor are only different because paraganglioma is outside adrenal and pheochromcytoma is inside the adrenal medulla

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6
Q

Granular cell tumor

A

S100+, CD68+

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7
Q

Congenital epulis

A

S100-

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8
Q

Hemangioma of infancy

A

GLUT1 +

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9
Q

Leiomyoma

A

MSA +, SMA +, desmin +

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10
Q

Liposarcoma

A

MDM2 +, CDK4+

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11
Q

Angiosarcoma

A

Factor 8 +, CD31 +, CD34+ FLI-1 +, ERG +

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12
Q

Kaposi sarcoma

A

HHV 8 + aka LANA-1 + (protein encoded by HHV8) ERG+ CD34+ CD31 + (some but not all) FLI-1 + (so HHV8 differentiates from angiosarc)

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13
Q

Rosai-Dorfman Disease

A

CD68+ CD163+ S100 + CD1a -

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14
Q

Secretory Carcinoma

A

Secretory Material: PAS Alcean Blue Mammaglobin Tumor cells Mammaglobin S100 STAT5a BRST2 (aka GCDFP15) CKs Myoepithelial markers are negative Molecular: t(12,15) ETV6-NTRK3

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15
Q

Neurofibroma

A

S100 + CD34 +

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16
Q

Pleomorphic Adenoma

A

Myoepithelial markers: p63 + SMA + Epithelial markers: CAM5.2 CK7 Genetic mutation: PLAG1 + Neuro / Dendritic markers: SOX10 + GFAP +

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17
Q

Melanoma

A

S100+ (spindle cell melanoma retains) Melan-A+ HMB45 + SOX10 + (spindle cell melanoma retains) Tyrosinase + MITF+

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18
Q

Small Round Blue Cell Tumor

A

MR SLEEP Melanoma (S100,SOX10,HMB45,Melan-A, etc) Rhabdomyosarcoma(Desmin,Myogenin, MyoD1) Sinonasal Undiff Ca (SNUC) (AE1/AE3, PanCK) Lymphoma (CD45, CD20, CD3) Ethesioneuroblastoma (olfactory) (S100, Synapto,Chromo,) Ewing Sarcoma (CD99, SMARCB1, FLI-1) Pituitary Adenoma (EGFR, Synapto,Chromo, Negative for most hormone markers (TSH,ACTH,LH,GH,FSH))

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19
Q

Langerhans Cell Histiocytosis

A

aka: eosinophilic granuloma (solitary) CD1a + S100 + Langerin + (AKA CD207)

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20
Q

Rosai-Dorfman

A

Aka: LN sinus histiocytosis with massive LAD CD68+ S100 + CD163 + CD1a - (emperipoesis on histo)

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21
Q

Osteosarcoma

A

MDM2 +

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22
Q

Plasma cell neoplasm

A

CD138+, CD79a+, Kappa / Lamda ISH

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23
Q

Ectomesenchymal Chondromyxoid tumor

A

GFAP +; RREB1-MKL2 fusion

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24
Q

Granulomatous inflammation

A

AFB, GMS, PAS

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25
DIF Patterns: Lupus Erythematosus
SHAGGY / GRANULAR band at basement membrane zone with IgM, IgG, C3
26
IIF Pattern: Wegner Granulomatosis
PR3-ANCA (Proteinase-3 antineutrophil cytoplasm antibodies, prev c-ANCA) and MPO-ANCA (myeloperoxidase, prev p-ANCA) directed against cytoplasmic components of neutrophils
27
IIF Pattern: Lichenoid drug reactions
string of pearls (annular pattern) along cell membrane of the basal cell layer with IgG
28
DIF Patterns: Pemphigus Vulgaris
"Chicken wire" IgG, IgM, C3 in intercellular spaces of epithelial cells
29
DIF Patterns: Paraneoplastic pemphigus
weakly positive IgG, C3 intercellular zones of the epithelium AND/OR a linear deposition at the basement membrane zone
30
DIF Patterns: Mucous membrane pemphigoid
linear band of immunoreactants )IgG, C3 at the basement membrane zone
31
DIF Patterns: Bullous pemphigoid
Continuous linear band of immunoreactants (IgG, C3) localized to the basement membrane zone
32
DIF Patterns: Erythema multiforme
Non-diagnostic (granular C3 + around blood vessels)
33
DIF Patterns: Lichen Planus
Fibrinogen, basement membrane zone
34
DIF: Chronic Ulcerative Stomatitis
"speckled" IgG in the basal and parabasal nuclei
35
Ewing sarcoma
CD99, FLI-1, SMARCB1
36
Acinic Cell Carcinoma
DOG1, SOX10, PASD+ , NR4A3+ (due to t(4,9)) PASD+ granules alpha-1-antichymotrypsin alpha amylase Myoepithelial markers: p63, p40, SMA
37
Adenoid Cystic Carcnoma
Biphasic (ducal and myoepithelial): Epithelial Markers: CAM5.2, CK7 Myoepithelial Markers: P63+, P40+ Others: CD43, CKIT
38
Polymorphous Adenocarcinoma
Monophasic (ducal proliferation only): Epithelial Markers: CAM5.2, CK7 Myoepithelial markers: P63+, P40-
39
Malignant peripheral nerve sheath tumor
S100, SOX10, h3k27me3
40
CEOT
Congo red +, apple green birefringence
41
Nodular Faciitis
MYH9-USP6 gene fusion SMA+ MSA+ Calponin + S100 - Desmin -
42
Pemphigus Vulgaris: "Chicken wire" IgG, IgM, C3 in intercellular spaces of epithelial cells
43
Pemphigus Vulgaris "Chicken wire" IgG, IgM, C3 in intercellular spaces of epithelial cells
44
Mucous membrane pemphigoid linear band of immunoreactants )IgG, C3 at the basement membrane zone
45
Mucous membrane pemphigoid linear band of immunoreactants )IgG, C3 at the basement membrane zone
46
Erythema multiforme Non-diagnostic (granular C3 + around blood vessels) Regezi: "EM: non-specific fine granular basement membrane zone fluorescence for C3"
47
Lichen Planus Fibrinogen (shaggy?), basement membrane zone Regezi: "BMZ and supericial LP fluor for fibrinogen and apoptotic body fluorescence for IgM"
48
Lichen Planus Fibrinogen (shaggy?), basement membrane zone Regezi: "BMZ and supericial LP fluor for fibrinogen and apoptotic body fluorescence for IgM"
49
Lupus Erythematosus SHAGGY / GRANULAR band at basement membrane zone with IgM, IgG, C3 Regezi :"course-granular BMZ for IgM"
50
Metastatic Renal Cell Carcinoma
PAX 8+, CD10 +, CAIX +
51
Metastatic Prostate Adenocarcinoma
Most common: NKX3.1, PSA, AMACR FOXA1+, HSD3B2+, ROCK1 PAP
52
Metastatic Breast, Invasive Ductal Carcinoma
MUC4, EMA, CK7, ER/PR, GATA3
53
Metastatic Lung Adenocarcinoma
CK7, NAPSIN-A, TTF-1
54
Metastatic Thyroid Carcinoma
TTF-1, Thyroglobulin, PAX8
55
Metastatic Colon Adenocarcinoma
CDX2, CK20, AMACR, CD10 (stromal cells)
56
Merkel Cell Carcinoma
CK20 (classic dot-like perinuclear), MCPyV, Chromo/Synapto, CD56, neuron specific enolase (NSE), Argyrophilic granules can be seen with Grimelius stain
57
Myopericytoma
SMA + muscle specific actin + h-caldesmon + CD34 -
58
Myofibroma
muscle specific actin (MSA/HHF35) + SMA + Desmin - pan-actin +
59
Inflammatory myofibroblastic tumor
POsitive: SMA, alpha smooth muscle actin, calponin, ALK
60
Glioma
GFAP
61
Sebaceous carcinoma
adipophilin to highight sebocytes EMA (not specific) LeuM1
62
Amorphous material in biopsy specimens of ligeneous gingivitis/conjunctivitis
Fraser-Lendrum IHC Negative for amyloid stains
63
Psedomyogenic hemangioendothelioma
FLI1+, **FOSB+**, CD34-, CD31+/-, ERG+, AE1/AE3+
64
Salivary duct carcinoma
Positive: Androgen receptor, HER2, EGFR, CK7, EMA (GATA3 nuclear) Focal + GCDFP-15 Negative: ER and PR Ki67 is high
65
Follicular lymphoma
GC markers: CD10+, BCL6+ BCL2+- normally stains the mantle zone but instead will be positive in the GC PAN B cell markers: CD79a, CD 19, CD20
66
Mantle cell lymphoma
CD43+, cyclin D1+, SOX11+, CD19+, CD20+, CD5+ GC markers are negative: BCL6- CD10-
67
Marginal zone lymphoma
CD19+, CD20+, BCL2+ GC markers neg: CD10-, BCL6- CD5-, cyclin D1-
68
CLL/SLL
Positive: CD79a, CD5, CD43, CD19, BCL2 Negative GC: CD10, BCL6 Negative: cyclin D1, SOX11
69
Tuberculosis
fluorescence microscopy of auramine-rhodamine stains Ziehl-Neelson AFB
70
Salivary lymphoepithelial carcinoma
CK, EBV, c-kit, EMA (all in carcinoma component) Surrounding lymphoid component is negative for these
71
Follicular cells of the thyroid
Thyroglobulin = most specific TTF-1 EMA, LMWK, EMA, ER beta, T3/T4
72
Colloid of the thyroid
thyroglobulin, Alcian blue, PAS, T3, T4
73
C cells of the thyroid
calcitonin, calcitonin gene related peptide (CGRP), neuron specific enolase, chromogranin A, synaptophysin, CEA, somatostatin C cells are metachromatic with toluidine blue NEGATIVE for thyroglobulin
74
Fite stain
Leprosy
75
Auramine-rhodamine
Florescent stain for acid-fast bacteria -mycobacteria tuberculosis
76
Ziehl-Neelson stains
acid fast bacteria aka mycobacteria
77
Fraser-Lendrum stains?
Fibrin of plasminogen deficiency
78
Amorphous material of lipoid proteinosis stain?
PAS+
79
Leishmaniasis
Giemsa
80
Grimelius stains?
intracytoplasmic granules of Merkel cell carcinoma
81
Epithelioid hemangioendohelioma
CD31+, FLI1+, ERG + Nuckear positivty with CAMTA1 in cases with corresponding fusion
82
Meningioma
EMA+, CK18+, ER+, PR+
83
NK T cell lymphoma
CD3, CD56, TIA1, granzyme B, perforin + EBER + CD4/5/8 -
84
Olfactory neuroblastoma
NSE, chromo, synapto, CD56 + CD99, p63, FLI1, CD45 -

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