Western blot

Question 1

1

Answer 1

Transfer from Gel to filter paper

Question 2

2

Answer 2

wash with blocking buffer to decrease non specific antibody binding

Question 3

3

Answer 3

incubate with primary AB

Question 4

4

Answer 4

Incubate with secondary AB and add substrate

Question 5

5

Answer 5

Enzyme product IDs location of target protein