biochem Flashcards

1
Q

Step 1 PC

A

immunize rabbit

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2
Q

2 PC

A

obtain blood sample

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3
Q

3 PC

A

purify antigen using affinity chromatography

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4
Q

Step 1 MC

A

Multiple immunizations

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5
Q

2 MC

A

isolate B cells and tumor cells

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6
Q

3 MC

A

Fuse B and tumor cells

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7
Q

4 MC

A

Two sample: antigen specific and non-specific hybridoma

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8
Q

5 MC

A

ID antigen specific by screening

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9
Q

6 MC

A

clone antigen specific

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10
Q

7 MC

A

results in monoclonal ABs

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