WEEK2 Flashcards
Techniques in Molecular Biology
What are some fundamental techniques in cellular and molecular biology
- cell lysis
- Fractional and chromatography techniques
- Electrophoresis
- Detection, Identification, Quantization of specific nucleic acids
-Transcriptomics - Isolating genes
- Polymerase Chain Reaction
- Reverse Transcription
- Gene editing
- Protein clevage
- Membrane Dynamics (FRAP)
What are some tools used in basic biology?
-Microscope
-NMR
-chromatography
-centrifuge
-refrigerator
What are some tools used in biotechnology?
-Gel Electrophoresis
-Gene sequencer
What are blotting techniques?
Blotting techniques are used to separate and identify molecules such as DNA, RNA and Proteins.
What is southern blotting?
Southern blotting is used to Analyse the genome of organisms
What is southern blotting ?
technique used to analyze gene expression by detecting DNA
What is western blotting?
the technique used to analyze and detect proteins
What is polymerase chain reaction?
technique used to copy a specific DNA segment millions of times
What is gel electrophoresis?
a technique used to separate DNA, RNA or proteins
enzyme-linked immunosorbent assays
used to detect the presence of specific antibodies or antigens in a sample.
What are some uses of PCR in medicine?
Diagnosis of infectious diseases, DNA fingerprinting, detection of bacteria or viruses, and diagnosis of genetic disorders
What is the first step of DNA analysis?
Extraction. It starts with DNA being extracted from a biological sample.
The technique used in DNA extraction often depends on..?
The technique chosen often depends on the size of the sample and the amount of DNA likely to be present.
What are the factors to consider in DNA samples?
- Obtaining a biological material (sample) for DNA analysis
- Incubation (cultivation) of biological material
- storage of biological material
- Avoid contamination - Disinfection, sterilization, decontamination,
#details in lecture 2A, 1
