Week 9 Notes Flashcards
Humoral immune system has to do with?
Antibodies
The innate immune system includes?
Skin, nasopharynx, gut, lungs, genitourinary tract, naturally occurring bacteria
What are the five Ig molecules?
IgG, IgM, IgE, IgA, IgD
Serology is used for?
Endocrinology testing, drug testing, and detecting infectious agents
What is the basic principle of serology?
Bind antigen to antibody and link that binding to a detection system.
What ELISA stand for?
Enzyme-linked Immunoabsorbent Assay
What does CELISA stand for?
Competitive enzyme-linked Immunoabsorbent assay
Basic principle of ELISA tests?
Bound groups react with the enzyme to produce a color change
Basic principle of CELISA tests?
Sample must compete with the antigen for binding sites to produce a color change
Basic principle of latex agglutination
Binding of matching antibodies and antigen cause agglutination
What does RIM stand for?
Rapid immunomigration
What is different about the RIM test?
Uses colloidal gold
Coombs indicates?
IMHA
Titters can be used to?
Measure infection or vaccine function
DNA testing is used to?
Classify cancers, detect genetic defects, verify pedigree, determine bacterial contaminants in food science applications.
Blood type is determined by?
Natural antigens on the surface of the RBC’s
What are the blood types for the cat and dog?
Dog- DEA
Cats- A, B, and AB groups
What is major and minor cross matching and where do we use it?
In large animals
Major- downer RBC’s and patient plasma
Miner- downer plasma and patient RBC’s
What are the blood collection measurements for dogs, cats, and horses?
Dog- 13-17 mL/kg
Cats- 11-15 mL/kg
Horses- 16 mL/kg
What anticoagulants do we use in blood that will be used immediately?
Sodium citrate
How many blood types for cattle and what is the most common?
11 known groups, j negative most common
How many blood groups do sheep and goats have?
Seven
How many blood groups do horses have?
More than 30
What are the different blood products?
Whole blood, packed RBC’s, plasma ( fresh of fresh frozen)
