Week 9 MC

Question 1

Step 1 of the CAC

Answer 1

Citrate Synthase adds an actyl group to oxaloacetate

Add 2 carbon-containing groups together
A 2 step mechanism
Hydration/Cleavage of Thioester bond drives this synthesis

Question 2

Step 2 of the CAC

Answer 2

Aconitate Isomerizes Citrate to Isocitrate

Not really an isomerase but a lyase

Question 3

Step 3 of the CAC

Answer 3

Isocitrate dehydrogenase catalyses the oxidateive decarboxylation of isocitrate forming alpha keto glutarate and NADH

alpha keto acid is ready to decarboxylate

Question 4

Step 4 of the CAC

Answer 4

Alpha Ketoglutarate dehydrogenase complex catalyzes the synthesis of sucinyl CoA from alpha ketoglutarate, generating NADH and CO2

Energy from decarboxylation used ot make high energy thioester bond

Multiple steps, resemble pyruvate dehydrogenase complex

Question 5

Step 5 of the CAC

Answer 5

Succinyl CoA synthetase catalyzes the cleaveage of a thioester linkages forms ATP

Makes ATP
Passing Phosphate 3 times to diff groups

Question 6

Steps 6,7,8

Answer 6

6 : Oxidation-Succinate Dehydrogenase Succinate to Fumerate
7: Addition-Fumerate
Fumerate to Malate
8: Oxidation-Malate Dehydrogenase
Malate to Oxaloacetate

Question 7

What are the Regulatory enzymes for the CAC

Answer 7

Steps 3: Isocitrate Dehydrogenase
Step 4: a-ketoglutarate dehydrogenase complex