Week 8

Question 1

What are cold AB

Answer 1

Clinically insignificant (dont cause red cell destruction) but interfere with testing intended to detect significant AB
-IgM AB present at IS, RT and sometimes 37
-can show MF
-to enhance the reaction incubate at RT or below, some are even incubated in the fridge

Question 2

how will you know you have a cold antibody

Answer 2

DAT positive
-polyspecific is pos and then you just have to differentiate between the IgG and C3
-consistent reactions in panels
-can use absorption to remove AB
-can be in a ABO ab
-pts can have history of anemia, mycoplasma pneumonia or infectious mononucleosis
-if blood products are needed then you may have to prewarm

Question 3

What is ANTI I

Answer 3

autoantibody is common , optimal reaction at cold temps
- not significant but reactivity varies because of the chain structures
-Anti I reacts with H = compound antibody
-ANTI IH has a strong agglutination with cells that have more H ag like O and A2
-Allo Anti-I is rare and Auto Anti-i is uncommon

Question 4

Auto Anti-I is associated with

Answer 4

Mycoplasma pneumoniae and CAD

Question 5

Anti-i is associated with

Answer 5

Infectious mononucleosis,
lymphoproliferative disease, and occasionally cold hemagglutinin
disease.

Question 6

cold allo-antibodies include:

cold auto antibodies include

Answer 6

Anti- P1, -M, -N, -𝐋EA and Leb

Anti-IH and Anti-I

Question 7

SUBGROUPS OF A

Answer 7

A1 sub group has A1 and A AG with (Anti B in plasma

A2 subgroup has A ag with Anti A and Anti B in plasma (there will be an extra reaction in the reverse cells)

confirm by phenotyping pt with Anti A1 lectin

Question 8

Avoid Anti-complement in the AHG test:

Answer 8

use Anti-IgG antiglobulin reagent
instead of Polyspecific reagent.

remove the readings at IS and 37

avoid LISS to enhance and use albumin instead with an increase in incubation

do the prewarming technique