Week 11

Question 1

What are the three components that blood products are separated into by centrifugation

Answer 1

Plt
RBC
PLASMA

Question 2

What are the different components that can be derived from blood products

Answer 2

• Red Blood Cells (RBC) - can be further modified
• Plasma (FP) - must be frozen within 24 hours
• Plasma (AFFP) - must be frozen within 8 hours
• Cryosupernatant Plasma (CSP)
• Cryoprecipitate
• Platelets
• Plasma Fractionation Products - IVIG
• Protein - Albumin 5% or Albumin 25%
• Coagulation factors, which can be a human plasma source or recombinant, are produced

Question 3

What is the universal leukocyte reduction method

Answer 3

-pre storage leukoreduction method
-leuks are removed from RBC and platelet portion before storage
* Pre-storage leukoreduction to a threshold of 5 X 106 per component can prevent most FNHTR
associated with RBC and platelet component transfusions

Question 4

What is the circular of information

Answer 4

• Extension of the Component label
  -states info on packaging , storage, and handling
  -warnings and precautions
  -dose and information
  -volumes and counts per bag

Question 5

how are Red Cell products stored

Answer 5

• Leukocyte Reduced (LR)
• SAGM (Additive) is added
• Expiry date is 42 days if the product is not modified
• Approximately 480 mL of whole blood collected with 70 mL of CPD anticoagulant
• RBC LR SAGM stored at 1 -6̊ C
• Refer to the Circular of information for Detailed product information

Question 6

What are the different types of plasma products

Answer 6

AFFP
FP
Octaplasma
cyroprecipitate
cryosupernatant plasma

Question 7

how is AFFP collected

Answer 7

-by apheresis and frozen in 8 hours of collection (have FV, FVIII and non labile coagulation factors)

Question 8

how is FP collected

Answer 8

-separated from whole blood and frozen in 24 hours of collection (low FV and FVIII)

Question 9

How is octaplasma collected

Answer 9

-solvent detergent treated plasma
-pooled fresh plasma is filtered so cells and debris are removed helps to reduce transfusion related lung injury

Question 10

what is cyroprecipitate

Answer 10

-has only the proteins that precipitate out after you freeze plasma during slow and cool thaw

Question 11

What is cryosupernatant plasma

Answer 11

has everything except the proteins that end up in the cyroprecipitate
stored at -18 or colder for a maximum period of 12 months

Question 12

What are two methods of producing plasma fractionation products

Answer 12

plasma derived (PD) from Cohn or Cold ethanol fractionation
-pooled plasma from female donors

Recombinant (R) uses biotechnology
-cultured cells produce and secrete proteins into a medium = collected and purified

Question 13

What is the whole blood without platelet retrieval method

Answer 13

IF only RBC and plasma were needed
-cool blood down to 1-6
-filter
-HARD SPIN
-remove plasma
-add SAGM to RBC
-process plasma

use CPD and SAGM

Question 14

What is the Buffy Coat Production Method
(BCPM)

Answer 14

IF platelets are needed
-plts need to be seperated from WB in 24 hours
-cool WB with rapid cooling at 18-24
-hard spin
-separate WB into 3 layers BC, Plasma and RBC
-leave only BC in bag
-Add SAGM to RBC and filter
-freeze plasma as FP or make into cyroprecipitate
-BC has WBC and PLT
-BC from 7 people and plasma from 1 pooled
-SOFT spin PRP separated and filtered to use for plts
-wbc discarded

use CPD and SAGM

Question 15

What does the label for blood collection bag contain

Answer 15

• Donor Center will label all units
• ABO/Rh
• Collection and Expiry Date
• Modifications and Volumes
• Special Typing, e.g., phenotyping
• Unit identifier
• Product Type

Question 16

Green colored plasma occurs when

Answer 16

when youve taken birth control pills

Question 17

if INR is normal

Answer 17

dont give plasma